Structure of PDB 8t76 Chain A Binding Site BS01

Receptor Information
>8t76 Chain A (length=1088) Species: 1314 (Streptococcus pyogenes) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
KKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALL
FDSGETAERTRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLR
LIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPIN
ASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNF
KSNFGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEH
HQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKP
ILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQED
FYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNF
EEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVK
YVTFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVED
RFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKS
DGFANRNFMQLIHDDSLTFKEDIQKAQVSSLHEHIANLAGSPAIKKGILQ
TVKVVDELVKVMGRHKPENIVIEMQITKHVAQILDSRMNTKYDENDKLIR
EVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKR
DFATVRKVLSMPQVNIVKKTEVQTGGFSKESIRPKRNSDKLIARKKDWDP
KKYGGFLWPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNP
IDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAKQLQKGNELAL
PSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSK
RVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTRLGAPRAFKYFD
TTIDPKQYRSTKEVLDATLIHQSITGLYETRIDLSQLG
Ligand information
>8t76 Chain B (length=88) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
gaugagacgcguuuuagagcuagaaauagcaaguuaaaauaaggcuaguc
cguuaucaacuugaaaaaguggcaccgagucggugcuu
..........<<<<<<..<<<<....>>>>....>>>>>>..<<.....>
>.......<<<<....>>>>.<<<<<<...>>>>>>..
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB8t76 Unraveling the mechanisms of PAMless DNA interrogation by SpRY-Cas9
Resolution3.42 Å
Binding residue
(original residue number in PDB)		K30 K33 L35 R40 I43 K44 N46 G56 E57 T58 A59 T62 R63 K65 R66 R69 R70 R71 Y72 R74 K76 N77 R78 Y81 L101 S104 F105 R115 H116 I122 E125 V126 H129 I135 H160 K163 F164 R165 G166 Y325 H328 H329 K336 F351 F352 Y359 A360 I363 Q402 R403 T404 F405 N407 G408 F446 R447 I448 Y450 G453 P454 L455 A456 R457 N459 S460 F462 W464 R467 I473 P475 N497 G729 K734 K735 K742 K749 Q926 M1089 P1090 Q1091 K1097 T1102 G1103 G1104 F1105 I1110 P1112 K1113 R1122 K1123 K1124 K1130 Y1131 M1169 E1225 A1227 Q1272 H1349 Q1350 S1351 I1352 Y1356 T1358
Binding residue
(residue number reindexed from 1)		K28 K31 L33 R38 I41 K42 N44 G54 E55 T56 A57 T60 R61 K63 R64 R67 R68 R69 Y70 R72 K74 N75 R76 Y79 L99 S102 F103 R113 H114 I120 E123 V124 H127 I133 H158 K161 F162 R163 G164 Y297 H300 H301 K308 F323 F324 Y331 A332 I335 Q374 R375 T376 F377 N379 G380 F418 R419 I420 Y422 G425 P426 L427 A428 R429 N431 S432 F434 W436 R439 I445 P447 N469 G690 K695 K696 K703 K710 Q725 M811 P812 Q813 K819 T824 G825 G826 F827 I832 P834 K835 R844 K845 K846 K852 Y853 M891 E947 A949 Q994 H1071 Q1072 S1073 I1074 Y1078 T1080
Enzymatic activity
Enzyme Commision number 3.1.-.-
Gene Ontology
Molecular Function
GO:0003676 nucleic acid binding
GO:0003677 DNA binding
GO:0003723 RNA binding
GO:0004519 endonuclease activity
GO:0004520 DNA endonuclease activity
GO:0004527 exonuclease activity
GO:0008408 3'-5' exonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0043571 maintenance of CRISPR repeat elements
GO:0051607 defense response to virus

View graph for
Molecular Function
View graph for
Biological Process
External links
PDB RCSB:8t76, PDBe:8t76, PDBj:8t76
PDBsum8t76
PubMed38688943
UniProtQ99ZW2|CAS9_STRP1 CRISPR-associated endonuclease Cas9/Csn1 (Gene Name=cas9)

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