Structure of PDB 8t6y Chain A Binding Site BS01

Receptor Information
>8t6y Chain A (length=1148) Species: 1314 (Streptococcus pyogenes) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
KKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALL
FDSGETAERTRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLR
LIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPIN
ASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNF
KSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILL
SDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFF
DQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQ
RTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYV
GPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNL
PNEKVLPKHSLLYEYFTVYNELTKVKYVTFLSGEQKKAIVDLLFKTNRKV
TVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDN
EENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGW
GRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQ
KAQVSSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEM
QITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKV
REINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGIMNFFKPLIETNGE
TGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESIRPKRNSDK
LIARKKDWDPKKYGGFLWPTVAYSVLVVAKVEKGKSKKLKSVKELLGITI
MERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAK
QLQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDE
IIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTRL
GAPRAFKYFDTTIDPKQYRSTKEVLDATLIHQSITGLYETRIDLSQLG
Ligand information
>8t6y Chain B (length=88) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
agaaauacgcguuuuagagcuagaaauagcaaguuaaaauaaggcuaguc
cguuaucaacuugaaaaaguggcaccgagucggugcuu
..........<<<<<<..<<<<....>>>>....>>>>>>..<<.....>
>.......<<<<....>>>>.<<<<<<...>>>>>>..
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB8t6y Unraveling the mechanisms of PAMless DNA interrogation by SpRY-Cas9
Resolution2.88 Å
Binding residue
(original residue number in PDB)		K30 K33 L35 R40 K44 N46 G56 E57 T58 A59 R61 T62 R63 K65 R66 R69 R70 R71 Y72 R74 R75 K76 N77 R78 Y81 L101 S104 F105 R115 H116 I122 E125 H129 I135 H160 K163 F164 R165 G166 Y325 H328 H329 K336 F351 F352 Y359 A360 I363 Q402 R403 T404 F405 N407 G408 F446 R447 I448 Y450 G453 P454 L455 A456 R457 N459 S460 F462 W464 R467 T472 I473 P475 M495 N497 P731 K742 R753 M1089 P1090 Q1091 K1097 E1099 V1100 T1102 G1103 G1104 F1105 I1110 P1112 R1122 K1123 K1124 Y1131 M1169 E1225 A1227 Q1272 H1349 Q1350 S1351 Y1356
Binding residue
(residue number reindexed from 1)		K28 K31 L33 R38 K42 N44 G54 E55 T56 A57 R59 T60 R61 K63 R64 R67 R68 R69 Y70 R72 R73 K74 N75 R76 Y79 L99 S102 F103 R113 H114 I120 E123 H127 I133 H158 K161 F162 R163 G164 Y323 H326 H327 K334 F349 F350 Y357 A358 I361 Q400 R401 T402 F403 N405 G406 F444 R445 I446 Y448 G451 P452 L453 A454 R455 N457 S458 F460 W462 R465 T470 I471 P473 M493 N495 P718 K729 R740 M871 P872 Q873 K879 E881 V882 T884 G885 G886 F887 I892 P894 R904 K905 K906 Y913 M951 E1007 A1009 Q1054 H1131 Q1132 S1133 Y1138
Enzymatic activity
Enzyme Commision number 3.1.-.-
Gene Ontology
Molecular Function
GO:0003676 nucleic acid binding
GO:0003677 DNA binding
GO:0003723 RNA binding
GO:0004519 endonuclease activity
GO:0004520 DNA endonuclease activity
GO:0004527 exonuclease activity
GO:0008408 3'-5' exonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0043571 maintenance of CRISPR repeat elements
GO:0051607 defense response to virus

View graph for
Molecular Function
View graph for
Biological Process
External links
PDB RCSB:8t6y, PDBe:8t6y, PDBj:8t6y
PDBsum8t6y
PubMed38688943
UniProtQ99ZW2|CAS9_STRP1 CRISPR-associated endonuclease Cas9/Csn1 (Gene Name=cas9)

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