Structure of PDB 8sfh Chain A Binding Site BS01

Receptor Information
>8sfh Chain A (length=1286) Species: 1111120 (Acidaminococcus sp. BV3L6) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDHYKEL
KPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQA
TYRNAIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVT
TTEHENALLRSFDKFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPK
FKENCHIFTRLITAVPSLREHFENVKKAIGIFVSTSIEEVFSFPFYNQLL
TQTQIDLYNQLLGGISKIKGLNEVLNLAIQKNDETAHIIASLPHRFIPLF
KQILSDSFILEEFKSDEEVIQSFCKYKTLLRNENVLETAEALFNELNSID
LTHIFISHKKLETISSALCDHWDTLRNALYERRISELKSAKEKVQRSLKH
EDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLKKQEEKE
ILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYN
KARNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYL
GIMPKQKGRYKALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAV
TAHFQTHTTPILLSNNFIEPLEITKEIYDLNNPEKEPKKFQTAYAKKTGD
QKGYREALCKWIDFTRDFLSKYTKTTSIDLSSLRPSSQYKDLGEYYAELN
PLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGHHGKPNLHTLYW
TGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKDQK
TPIPDTLYQELYDYVNHRDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVID
STGKILEQRSLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGY
LSQVIHEIVDLMIHYQAVVVLENLNFGFKSKRTGIAEKAVYQQFEKMLID
KLNCLVLKDYPAEKVGGVLNPYQLTDQFTSFAKMGTQSGFLFYVPAPYTS
KIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDVKTGDFILHFKMNR
NLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENHRFTG
RYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRS
VLQMRNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIA
LKGQLLLNHLKESKDLKLQNGISNQDWLAYIQELRN
Ligand information
>8sfh Chain B (length=27) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
aauuucuacucuuguagaugugauaag
....<<<<<....>>>>>.........
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB8sfh WT CRISPR-Cas12a with a 5bp R-loop
Resolution3.4 Å
Binding residue
(original residue number in PDB)		S14 K15 T16 R18 F19 Y47 K51 N175 K524 Y526 K530 N747 K748 G753 H755 G756 K757 N759 L760 H761 E786 R790 H800 M806 L807 N808 K809 K810 K852 H856 I858 I859 K860 D861 R862 R863 F870 H872 Q936 F938 K943 G970 S973 Q974 H977 K1022 K1029
Binding residue
(residue number reindexed from 1)		S14 K15 T16 R18 F19 Y47 K51 N175 K509 Y511 K515 N732 K733 G738 H740 G741 K742 N744 L745 H746 E771 R775 H785 M791 L792 N793 K794 K795 K831 H835 I837 I838 K839 D840 R841 R842 F849 H851 Q915 F917 K922 G949 S952 Q953 H956 K1001 K1008
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003723 RNA binding
GO:0004519 endonuclease activity
GO:0004530 deoxyribonuclease I activity
GO:0016829 lyase activity
Biological Process
GO:0006259 DNA metabolic process
GO:0051607 defense response to virus

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Molecular Function
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Biological Process
External links
PDB RCSB:8sfh, PDBe:8sfh, PDBj:8sfh
PDBsum8sfh
PubMed
UniProtU2UMQ6|CS12A_ACISB CRISPR-associated endonuclease Cas12a (Gene Name=cas12a)

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