Structure of PDB 8rc0 Chain A Binding Site BS01

Receptor Information
>8rc0 Chain A (length=2153) Species: 9606 (Homo sapiens) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
KEDMPPEHVRKIIRDHKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPV
LYHITGAISFVNEIPWVIEPVYISQWGSMWIMMRREKRDRRHFKRMRFPP
FDDEEPPLDYADNILDVEPLEAIQLELDPEEDAPVLDWFYDHQPLRDSRK
YVNGSTYQRWQFTLPMMSTLYRLANQLLTDLVDDNYFYLFDLKAFFTSKA
LNMAIPGGPKFEPLVRDINLQDEDWNEFNDINKIIIRQPIRTEYKIAFPY
LYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPISHREPLPDD
DEEFELPEFVEPFLKDTPLYTDNTANGIALLWAPRPFNLRSGRTRRALDI
PLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFR
SFKATKFFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNL
KPVKTLTTKERKKSRFGNAFHLCREVLRLTKLVVDSHVQYRLGNVDAFQL
ADGLQYIFAHVGQLTGMYRYKYKLMRQIRMCKDLKHLIYYRFNTGPVGKG
PGCGFWAAGWRVWLFFMRGITPLLERWLGNLLAHFDLELRAAVMHDILDM
MPEGIKQNKARTILQHLSEAWRCWKANIPWKVPGLPTPIENMILRYVKAK
ADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLTRLYLKAEQERQHNYLK
DGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLILALERL
KEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGI
EFMDLYSHLVPVYDVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTE
PPPLLVYKWCQGINNLQDVWETSEGECNVMLESRFEKMYEKIDLTLLNRL
LRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGIIRGLQFASFIVQYYGL
VMDLLVLGLHRASEMAGPPQMPNDFLSFQDIATEAAHPIRLFCRYIDRIH
IFFRFTADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARMRLMKHD
VNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRI
LPKCRTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQ
ILMASGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQ
TRIKIGLNSKMPSRFPPVVFYTPKELGGLGMLSMGHVLIPQSDLRWSKQT
DVGITHFRSQLIPNLYRYIQPWESEFIDSQRVWAEYALKRQEAIAQNRRL
TLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTDFKQYQVLKQNPF
WWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEGLFW
EKPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIF
RAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSYKMNSSCA
DILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIE
RYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQ
QAMAKIMKANPALYVLRERIRKGLQLYEPYLSSQNYGELFSNQIIWFVDD
TNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKR
LGQLAKWKTAEEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNI
VIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLYDDWLKTISSYTA
FSRLILILRALHVNNDRAKVILKPDKTTITEPHHIWPTLTDEEWIKVEVQ
LKDLILADYGKKNNVNVASLTQSEIRDIILGMEIFSSKTEWRVRAISAAN
LHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICISDLRAQIAGYLYGVS
PPDNPQVKEIRCIVMVPQWGTHQTVHLPGQLPQHEYLKEMEPLGWIHTQP
NESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTP
SGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSWNYN
FMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADRED
LYA
Ligand information
>8rc0 Chain 5 (length=104) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
acucugguuucucuucagaucgcauaaaucuuucgcacuaaagauuuccg
uggagaggaacaacucugaguccaauuuuuugaggccuugcuuuggcaag
gcua
<<<<.<<<<<<<<<<<<........<<<<<<<<......>>>>>>>>...
>>>>>>>>>>>....>.>>>>............<<<<<<<<....>>>>>
>>>.
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB8rc0 Structure of the human 20S U5 snRNP.
Resolution3.2 Å
Binding residue
(original residue number in PDB)		H97 I132 W134 R217 N221 G222 T224 F279 E280 R417 R419 R420 D423 K428 K452 N457 H461 P463 P464 K465 A466 Q467 K468 K469 R470 R474 K595 Y596 K597 M599 Q601 R603 R635 F639 F640 R642 G643 P646
Binding residue
(residue number reindexed from 1)		H29 I64 W66 R149 N153 G154 T156 F211 E212 R343 R345 R346 D349 K354 K378 N383 H387 P389 P390 K391 A392 Q393 K394 K395 R396 R400 K521 Y522 K523 M525 Q527 R529 R561 F565 F566 R568 G569 P572
Enzymatic activity
Enzyme Commision number ?
Gene Ontology
Molecular Function
GO:0003723 RNA binding
GO:0005515 protein binding
GO:0008233 peptidase activity
GO:0008237 metallopeptidase activity
GO:0017070 U6 snRNA binding
GO:0030619 U1 snRNA binding
GO:0030620 U2 snRNA binding
GO:0030623 U5 snRNA binding
GO:0070530 K63-linked polyubiquitin modification-dependent protein binding
GO:0097157 pre-mRNA intronic binding
GO:0140492 metal-dependent deubiquitinase activity
Biological Process
GO:0000244 spliceosomal tri-snRNP complex assembly
GO:0000375 RNA splicing, via transesterification reactions
GO:0000398 mRNA splicing, via spliceosome
GO:0006397 mRNA processing
GO:0008380 RNA splicing
GO:0071222 cellular response to lipopolysaccharide
GO:0071356 cellular response to tumor necrosis factor
Cellular Component
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005681 spliceosomal complex
GO:0005682 U5 snRNP
GO:0016020 membrane
GO:0016607 nuclear speck
GO:0030532 small nuclear ribonucleoprotein complex
GO:0046540 U4/U6 x U5 tri-snRNP complex
GO:0071005 U2-type precatalytic spliceosome
GO:0071006 U2-type catalytic step 1 spliceosome
GO:0071007 U2-type catalytic step 2 spliceosome
GO:0071013 catalytic step 2 spliceosome

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8rc0, PDBe:8rc0, PDBj:8rc0
PDBsum8rc0
PubMed38467877
UniProtQ6P2Q9|PRP8_HUMAN Pre-mRNA-processing-splicing factor 8 (Gene Name=PRPF8)

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