Structure of PDB 8r6u Chain A Binding Site BS01

Receptor Information

>8r6u Chain A (length=1566) Species: 992210 (SFTS virus AH12)

MNLEVLCGRINVENGLSLGEPGLYDQIYDRPGLPDLDVTVDATGVTVDIG
AVPDSASQLGSSINAGLITIQLSEAYKINHDFTFSGLSKTTDRRLSEVFP
ITHDGSDGMTPAVIHTRLDGTIVVVEFSTTRSHNIGGLEAAYRTKIEKYR
DPISRRVDIMENPRVFFGVIVVSSGGVLSNMPLTQDEAEELMYRFCIANE
IYTKARSMDADIELQKSEEELEAISRALSFFSLFEPNIERVEGTFPNSEI
KMLEQFLSTPADVDFITKTLKAKEVEAYADLCDSHYLKPEKTIQERLEIN
RCEAIDKTQDLLAGLHARSNKQTSLNRGTVKLPPWLPKPSSESIDIKTDS
GFGSLMDHGAYGELWAKCLLDVSLGNVEGVVSDPAKELDIAISAKITYRR
FKPALSSSARQEFSLQGVEGKKWKRMAANQKKEKESHETLSPFLDVEDIG
DFLTFNNLLTDSRYGDESIQRAVSILLEKASAMQDTELTHALNDSFKRNL
SSNVVQWSLWVSCLAQELASALKQHCRAGEFIIKKLKFWPIYVIIKPTKS
SSHIFYSLGIRKADVTRRLTGRVFSDTIDAGEWELTEFKSLKTCKLTNLV
NLPCTMLNSIAFWREKLGVAPWLVRKPCSELREQVGLTFLISLEDKSKTE
EIITLTRYTQMEGFVSPPMLPKPQKMLGKLDGPLRTKLQVYLLRKHLDCM
VRIASQPFSLIPREGRVEWGGTFHAISGRSTNLENMVNSWYIGYYKNKEE
STELNALGEMYKKIVEMEEDKPSSPEFLGWGDTDSPKKHEFSRSFLRAAC
SSLEREIAQRHGRQWKQNLEERVLREIGTKNILDLASMKATSNFSKDWEL
YSEVQTKEYHRSKLLEKMATLIEKGVMWYIDAVGQAWKAVLDDGCMRICL
FKKNQHGGLREIYVMDANARLVQFGVETMARCVCELSPHETVANPRLKNS
IIENHGLKSARSLGPGSININSSNDAKKWNQGHYTTKLALVLCWFMPAKF
HRFIWAAISMFRRKKMMVDLRFLAHLSSKSESRSSDPFREAMTDAFHGNR
DVSWMDKGRTYIKTETGMMQGILHFTSSLLHSCVQSFYKSYFVSKLKEGY
MGESISGVVDVIEGSDDSAIMISIRPKSDMDEVRSRFFVANLLHSVKFLN
PLFGIYSSEKSTVNTVYCVEYNSEFHFHRHLVRPTLRWIAASHQISETEA
LASRQEDYSNLLTQCLEGGASFSLTYLIQCAQLLHHYMLLGLCLHPLFGT
FMGMLISDPDPALGFFLMDNPAFAGGAGFRFNLWRACKTTDLGRKYAYYF
NEIQGSVMVYWGDRKKYQALLNRMGLPEDWVEQIDENPGVLYRRAANKKE
LLLKLAEKVHSPGVTSSLSKGHVVPRVVAAGVYLLSRHCFRFSKASLIKL
LMMSSISAMKHGGSLNPNQERMLFPQAQEYDRVCTLLEEVEHLTGKFVVR
ERNIVRSRIDLFQEPVDLRCKAEDLVSEVWFGLKRTKLGPRLLKEEWDKL
RASFAWLSTDPSETLRDGPFLSHVQFRNFIAHVDAKSRSVRLLGAPVVTT
ISQVVRMNFFPGFSLE

Ligand information

>8r6u Chain P (length=16)

acacagagacgcccag

.<.....>........

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8r6u Structural snapshots of phenuivirus cap-snatching and transcription
• Resolution: 2.98 Å
• Binding residue (original residue number in PDB): Q426 G427 K431 S446 H447 T558 K559 S562 H563 S600 K605 K656 R695 E763 N765 K1039 S1044 S1045 F1048 R1049
• Binding residue (residue number reindexed from 1): Q416 G417 K421 S436 H437 T548 K549 S552 H553 S590 K595 K646 R685 E753 N755 K1029 S1034 S1035 F1038 R1039

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.

Gene Ontology

Molecular Function

• GO:0003968 RNA-dependent RNA polymerase activity
• GO:0016740 transferase activity
• GO:0016787 hydrolase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0001172 RNA-templated transcription
• GO:0006351 DNA-templated transcription
• GO:0019079 viral genome replication
• GO:0039694 viral RNA genome replication

Cellular Component

• GO:0044165 host cell endoplasmic reticulum
• GO:0044172 host cell endoplasmic reticulum-Golgi intermediate compartment
• GO:0044177 host cell Golgi apparatus
• GO:0044423 virion component

External links

• PDB: RCSB:8r6u, PDBe:8r6u, PDBj:8r6u
• PDBsum: 8r6u
• PubMed: 38709882
• UniProt: I0DF35|L_SFTS RNA-directed RNA polymerase L