Structure of PDB 8pjj Chain A Binding Site BS01

Receptor Information
>8pjj Chain A (length=946) Species: 7227 (Drosophila melanogaster) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
TIENAKERLNIYKQTNNIRDDYKYTPVGPEHARSFLAELSIYVPALNRTV
TARESGSNKKSASKSCALSLVRQLFHLNVIEPFSGTLKKKKDEQLKPYPV
KLSPNLINKIDEVIKGLDLPVVNPESSVIPWAPPQANWNTWHACNIDEGE
LATTSIDDLSMDYERSLRDRRQNDNEYRQFLEFREKLPIAAMRSEILTAI
NDNPVVIIRGNTGCGKTTQIAQYILDDYICSGQGGYANIYVTQPRRISAI
SVAERVARERCEQLGDTVGYSVRFESVFPRPYGAILFCTVGVLLRKLEAG
LRGVSHIIVDEIHERDVNSDFLLVILRDMVDTYPDLHVILMSATIDTTKF
SKYFGICPVLEVPGRAFPVQQFFLEDIIQMTDFVPSAESEDKDEAEINYN
KVCEDKYSQKTRNAMAMLSESDVSFELLEALLMHIKSKNIPGAILVFLPG
WNLIFALMKFLQNTNIFGDTSQYQILPCHSQIPRDEQRKVFEPVPEGVTK
IILSTNIAETSITIDDIVFVIDICKARMKLFTSHNNLTSYATVWASKTNL
EQRKGRAGRVRPGFCFTLCSRARFQALEDNLTPEMFRTPLHEMALTIKLL
RLGSIHHFLSKALEPPPVDAVIEAEVLLREMRCLDANDELTPLGRLLARL
PIEPRLGKMMVLGAVFGCADLMAIMASYSSTFSEVFSLDIGQRRLANHQK
ALSGTKCSDHVAMIVASQMWRREKQRGEHMEARFCDWKGLQMSTMNVIWD
AKQQLLDLLQQAGFPEECMISHEVDERIDGDDPVLDVSLALLCLGLYPNI
CVHKEKRKVLTTESKAALLHKTSVNCSNLAVTFPYPFFVFGEKIRTRAVS
CKQLSMVSPLQVILFGSRKIDLAANNIVRVDNWLNFDIEPELAAKIGALK
PALEDLITVACDNPSDILRLEEPYAQLVKVVKDLCVKSAGDFGLQR
Ligand information
>8pjj Chain B (length=40) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
guguaaaauguugcuagcaugcuaguaacguuuuacgccc
..<<<<<.<<<<<<<<<<..>>>>>>>>>>>>>.>>....
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB8pjj Cryo-EM structure of MLE in complex with SL7UUC RNA and ADP
Resolution4.24 Å
Binding residue
(original residue number in PDB)		I167 E168 K171 E195 H196 R198 F200 S222 N223 K224 K229 T251 L252 L1030 K1033
Binding residue
(residue number reindexed from 1)		I2 E3 K6 E30 H31 R33 F35 S57 N58 K59 K64 T86 L87 L818 K821
Enzymatic activity
Enzyme Commision number 3.6.4.13: RNA helicase.
Gene Ontology
Molecular Function
GO:0001069 regulatory region RNA binding
GO:0003676 nucleic acid binding
GO:0003678 DNA helicase activity
GO:0003682 chromatin binding
GO:0003690 double-stranded DNA binding
GO:0003723 RNA binding
GO:0003724 RNA helicase activity
GO:0003725 double-stranded RNA binding
GO:0004386 helicase activity
GO:0005524 ATP binding
GO:0016787 hydrolase activity
GO:0016887 ATP hydrolysis activity
GO:0033679 3'-5' DNA/RNA helicase activity
GO:0034458 3'-5' RNA helicase activity
GO:0043138 3'-5' DNA helicase activity
GO:0106222 lncRNA binding
Biological Process
GO:0007549 sex-chromosome dosage compensation
GO:0008340 determination of adult lifespan
GO:0009047 dosage compensation by hyperactivation of X chromosome
GO:0010468 regulation of gene expression
GO:0031453 positive regulation of heterochromatin formation
GO:0032508 DNA duplex unwinding
GO:0042714 dosage compensation complex assembly
GO:0045433 male courtship behavior, veined wing generated song production
GO:0045944 positive regulation of transcription by RNA polymerase II
GO:0048675 axon extension
GO:0050684 regulation of mRNA processing
GO:2000373 positive regulation of DNA topoisomerase (ATP-hydrolyzing) activity
GO:2000765 regulation of cytoplasmic translation
Cellular Component
GO:0000228 nuclear chromosome
GO:0000785 chromatin
GO:0000805 X chromosome
GO:0005634 nucleus
GO:0005694 chromosome
GO:0005730 nucleolus
GO:0005829 cytosol
GO:0016456 X chromosome located dosage compensation complex, transcription activating
GO:0072487 MSL complex
GO:1990904 ribonucleoprotein complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8pjj, PDBe:8pjj, PDBj:8pjj
PDBsum8pjj
PubMed37989319
UniProtP24785|MLE_DROME Dosage compensation regulator mle (Gene Name=mle)

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