Structure of PDB 8pj9 Chain A Binding Site BS01

Receptor Information
>8pj9 Chain A (length=1051) Species: 1268061 (Streptococcus thermophilus DGCC 7710) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
KPYSIGLDIGTNSVGWAVITDNYKVPSKKMKVLGNTSKKYIKKNLLGVLL
FDSGITAEGRRLKRTARRRYTRRRNRILYLQEIFSTEMATLDDAFFQRLD
DSFLVPDDKRDSKYPIFGNLVEEKVYHDEFPTIYHLRKYLADSTKKADLR
LVYLALAHMIKYRGHFLIEGEFNVTDNETEAPLSSAMIKRYNEHKEDLAL
LKEYIRNISLKTYNEVFKDDTKNGYAGYIDGKTNQEDFYVYLKNLLAEFE
GADYFLEKIDREDFLRKQRTFDNGSIPYQIHLQEMRAILDKQAKFYPFLA
KNKERIEKILTFRIPYYVGPLARGNSDFAWSIRKRNEKITPWNFEDVIDK
ESSAEAFINRMTSFDLYLPEEKVLPKHSLLYETFNVYNELTKVRFIAESM
RDYQFLDSKQKKDIVRLYFKDKRKVTDKDIIEYLHAIYGYDGIELKGIEK
QFNSSLSTYHDLLNIINDKEFLDDSSNEAIIEEIIHTLTIFEDREMIKQR
LSKFENIFDKSVLKKLSRRHYTGWGKLSAKLINGIRDEKSGNTILDYLID
DGISNRNFMQLIHDDALSFKKKIQKAQIIGDEDKGNIKEVVKSLPGSPAI
KKGILQSIKIVDELVKVMGGRKPESIVVEMATKHVARLLDEKFNNKKDEN
NRAVRTVKIITLKSTLVSQFRKDFELYKVREINDFHHAHDAYLNAVIASA
LLKKYPKLEPEFVYGSNIMNIFKKSRPLIEVNEETGESVWNKESDLATVR
RVLSYPQVNVVKKVEEQNHGLDRGKPKGLFNANLSSKPKPNSNENLVGAK
EYLDPKKYGGYAGISNSFAVLVKGTIEKGAKKKITNVLEFQGISILDRIN
YRKDKLNFLLEKGYKDIELIIELPKYSLFELSDGSRRMLASILSTNNKRG
EIHKGNQIFLSQKFVKLLYHAKRISNTINENHRKYVENHKKEFEELFYYI
LEFNENYVGAKKNGKLLNSAFQSWQNHSIDELCSSFIGPTGSERKGLFEL
TSRGSAADFEFLGVKIPRYRDYTPSSLLKDATLIHQSVTGLYETRIDLAK
L
Ligand information
>8pj9 Chain B (length=36) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
cgcuaaagaggaagaggacaguuuuagagcuguguu
....................................
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB8pj9 Structural basis for spacer acquisition in a type II-A CRISPR-Cas system
Resolution3.24 Å
Binding residue
(original residue number in PDB)		T58 A59 R63 R66 R70 R71 R74 F105 P108 K111 K115 E125 K126 H129 Y164 R165 R403 T404 F405 R447 I448 Y450 P454 W464 Y501 Y515 N519 K660 M693 N1117 L1118 S1119 K1121
Binding residue
(residue number reindexed from 1)		T56 A57 R61 R64 R68 R69 R72 F103 P106 K109 K113 E123 K124 H127 Y162 R163 R269 T270 F271 R313 I314 Y316 P320 W330 Y367 Y381 N385 K526 M559 N783 L784 S785 K787
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003723 RNA binding
GO:0004519 endonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0043571 maintenance of CRISPR repeat elements
GO:0051607 defense response to virus

View graph for
Molecular Function
View graph for
Biological Process
External links
PDB RCSB:8pj9, PDBe:8pj9, PDBj:8pj9
PDBsum8pj9
PubMed
UniProtG3ECR1|CAS9_STRTR CRISPR-associated endonuclease Cas9 (Gene Name=cas9)

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