Structure of PDB 8p4e Chain A Binding Site BS01

Receptor Information
>8p4e Chain A (length=1407) Species: 9823 (Sus scrofa) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
SACPLRTIKRVQFGVLSPDELKRMSVTEGGIKYPETTEGGRPKLGGLMDP
RQGVIERTGRCQTCAGNMTECPGHFGHIELAKPVFHVGFLVKTMKVLRCV
CFFCSKLLVDSNNPKIKDILAKSKGQPKKRLTHVYDLCKGKNGGCGRYQP
RIRRSGLELYAEWKKKILLSPERVHEIFKRISDEECFVLGMEPRYARPEW
MIVTVLPVPPLSVRPAVRNQDDLTHKLADIVKINNQLRRNEQNGAAAHVI
AEDVKLLQFHVATMVDNELPGLPRAMQKSGRPLKSLKQRLKGKEGRVRGN
LMGKRVDFSARTVITPDPNLSIDQVGVPRSIAANMTFAEIVTPFNIDRLQ
ELVRRGNSQYPGAKYIIRDNGDRIDLRFHPKPSDLHLQTGYKVERHMCDG
DIVIFNRQPTLHKMSMMGHRVRILPWSTFRLNLSVTTPYNADFDGDEMNL
HLPQSLETRAEIQELAMVPRMIVTPQSNRPVMGIVQDTLTAVRKFTKRDV
FLERGEVMNLLMFLSTWDGKVPQPAILKPRPLWTGKQIFSLIIPGHINCI
RTHSTHPDDEDSGPYKHISPGDTKVVVENGELIMGILCKKSLGTSAGSLV
HISYLEMGHDITRLFYSNIQTVINNWLLIEGHTIGIGDSIADSKTYQDIQ
NTIKKAKQDVIEVIEKAHNNELEPTPGNTLRQTFENQVNRILNDARDKTG
SSAQKSLSEYNNFKSMVVSGAKGSKINISQVIAVVGQQNVEGKRIPFGFK
HRTLPHFIKDDYGPESRGFVENSYLAGLTPTEFFFHAMGGREGLIDTAVK
TAETGYIQRRLIKSMESVMVKYDATVRNSINQVVQLRYGEDGLAGESVEF
QNLATLKPSNKAFEKKFRFDYTNERALRRTLQEDLVKDVLSNAHIQNELE
REFERMREDREVLRVIFPTGDSKVVLPCNLLRMIWNAQKIFHINPRLPSD
LHPIKVVEGVKELSKKLVIVNGDDPLSRQAQENATLLFNIHLRSTLCSRR
MAEEFRLSGEAFDWLLGEIESKFNQAIAHPGEMVGALAAQSLGEPATQMT
LNTFHYAGVSAKNVTLGVPRLKELINISKKPKTPSLTVFLLGQSARDAER
AKDILCRLEHTTLRKVTANTAIYYDPNPQSTVVAEDQEWVNVISPWLLRV
ELDRKHMTDRKLTMEQIAEKINAGFGDDLNCIFNDDNAEKLVLRIRIMND
DDVFLRCIESNMLTDMTLQGIEQISKVYMHLPQTDNKKKIIITEDGEFKA
LQEWILETDGVSLMRVLSEKDVDPVRTTSNDIVEIFTVLGIEAVRKALER
ELYHVISFDGSYVNYRHLALLCDTMTCRGHLMAITRHGVNRQDTGPLMKC
SFEETVDVLMEAAAHGESDPMKGVSENIMLGQLAPAGTGCFDLLLDAEKC
KYGMEIP
Ligand information
>8p4e Chain P (length=21) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
ggacauacauaaagaccaggc
.....................
Receptor-Ligand Complex Structure
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PDB8p4e Structural insights into human co-transcriptional capping.
Resolution3.9 Å
Binding residue
(original residue number in PDB)		T68 N272 R334 F431 R460 D499
Binding residue
(residue number reindexed from 1)		T58 N219 R281 F378 R407 D446
Enzymatic activity
Enzyme Commision number 2.7.7.48: RNA-directed RNA polymerase.
2.7.7.6: DNA-directed RNA polymerase.
3.1.13.-
Gene Ontology
Molecular Function
GO:0001055 RNA polymerase II activity
GO:0003677 DNA binding
GO:0003899 DNA-directed 5'-3' RNA polymerase activity
GO:0016779 nucleotidyltransferase activity
GO:0016787 hydrolase activity
GO:0034062 5'-3' RNA polymerase activity
GO:0046872 metal ion binding
GO:1990841 promoter-specific chromatin binding
Biological Process
GO:0006351 DNA-templated transcription
GO:0006366 transcription by RNA polymerase II
GO:0033120 positive regulation of RNA splicing
Cellular Component
GO:0000428 DNA-directed RNA polymerase complex
GO:0000974 Prp19 complex
GO:0005634 nucleus
GO:0005665 RNA polymerase II, core complex
GO:0005694 chromosome
GO:0005737 cytoplasm
GO:0031981 nuclear lumen
GO:0032991 protein-containing complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8p4e, PDBe:8p4e, PDBj:8p4e
PDBsum8p4e
PubMed37369200
UniProtP11414|RPB1_CRIGR DNA-directed RNA polymerase II subunit RPB1 (Gene Name=POLR2A)

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