Structure of PDB 8i54 Chain A Binding Site BS01

Receptor Information
>8i54 Chain A (length=1113) Species: 1408323 (Lachnospiraceae bacterium MA2020) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
YESLTKQYPVSKTIRNELIPIGKTLDNIRQNNILESDVKRKQNYEHVKGI
LDEYHKQLINEALDNCTLPSLKIAAEIYLKNQKEVSDREDFNKTQDLLRK
EVVEKLKAHENFTKIGKKDILDLLEKLPSISEDDYNALESFRNFYTYFTS
YNKVRENLYSDKEKSSTVAYRLINENFPKFLDNVKSYRFVKTAGILADGL
GEEEQDSLFIVETFNKTLTQDGIDTYNSQVGKINSSINLYNQKNQKANGF
RKIPKMKMLYKQILSDREESFIDEFQSDEVLIDNVESYVKNDLAKTAMSN
IVFENWRTFDDLLNQEYDLANENKKKDDSCNLIENYIHQISDDIENILAK
NRKAVKAIKDFLDSIKVLERELKLINSSGQELEKVELKQVDSLYNMTRNY
LTKKPFSTEKVKLNFNRSTLLNGWDRNKETDNLGVLLLKDGKYYLGIMNT
SANKAFVNPPVAKTEKVFKKVDYKLLPVPNQMLPKVFFAKSNIDFYNPSS
EIYSNYKKGTHKKGNMFSLEDCHNLIDFFKESISKHEDWSKFGFKFSDTA
SYNDISEFYREVEKQGYKLTYTDIDETYINDLIERNELYLFQIYNKDFSM
YSKGKLNLHTLYFMMLFDQRNIDDVVYKLNGEAEVFYRPASISEDELIIH
KAGEEIKNKNPNRARTKETSTFSYDIVKDKRYSKDKFTLHIPITMNFGVD
EVKRFNDAVNSAIRIDENVNVIGIDRGERNLLYVVVIDSKGNILEQISLN
SIINKEYDIETDYHALLDEREGGRDKARKDWNTVENIRDLKAGYLSQVVN
VVAKLVLKYNAIICLEDLNFGFKRGRQKVEKQVYQKFEKMLIDKLNYIDK
SREQTSPKELGGALNALQLTSKFKSFKELGKQSGVIYYVPAYLTSKIDPT
TGFANLFYMKCENVEKSKRFFDGFDFIRFNALENVFEFGFDYRSFTQRAC
GINSKWTVCTNGERIIKYRNPDKNNMFDEKVVVVTDEMKNLFEQYKIPYE
DGRNVKDMIISNEEAEFYRRLYRLLQQTLQMRNSTSDGTRDYIISPVKNK
REAYFNSELSDGSVPKDADANGAYNIARKGLWVLEQIRQKSEGEKINLAM
TNAEWLEYAQTHL
Ligand information
>8i54 Chain B (length=33) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
aauuucuacuaauuguagaugccgcuaccccga
....<<<<<.....>>>>>..............
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB8i54 Structures of apo Cas12a and its complex with crRNA and DNA reveal the dynamics of ternary complex formation and target DNA cleavage.
Resolution3.95 Å
Binding residue
(original residue number in PDB)		K14 T15 R17 E19 Y153 K164 K166 S167 S168 K263 P495 F496 N685 K686 Y691 S692 K693 G694 K695 H699 R728 I746 R753 F762 D765 V767 K768 D769 R771 H780 N844 Y847 N890 K934 Y937
Binding residue
(residue number reindexed from 1)		K12 T13 R15 E17 Y151 K162 K164 S165 S166 K261 P405 F406 N595 K596 Y601 S602 K603 G604 K605 H609 R638 I656 R663 F672 D675 V677 K678 D679 R681 H690 N754 Y757 N800 K844 Y847
External links