Structure of PDB 8i0t Chain A Binding Site BS01

Receptor Information
>8i0t Chain A (length=2242) Species: 9606 (Homo sapiens) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
MSEEKLQEKARKWQQLQAKRYAEKRKFGFVQKEDMPPEHVRKIIRDHGDM
TNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLYHITGAI
SFVNEIPWVIEPVYISQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPL
DYADNILDVEPLEAIQLELDPEEDAPVLDWFYDHQPLRDSRKYVNGSTYQ
RWQFTLPMMSTLYRLANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGG
PKFEPLVRDWNEFNDINKIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYH
TPNVVFIKTEDPDLPAFYFDPLINPISHRHSVKSQEPLPDDDEEFELPEF
VEPFLKDTPLYTDNTANGIALLWAPRPFNLRSGRTRRALDIPLVKNWYRE
HCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATKFFQ
STKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTK
ERKKSRFGNAFHLCREVLRLTKLVVDSHVQYRLGNVDAFQLADGLQYIFA
HVGQLTGMYRYKYKLMRQIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAAG
WRVWLFFMRGITPLLERWLGNLLARQFEGRHSKGVAKTVTKQRVESHFDL
ELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCWKANIPWKVPGLPT
PIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLTRLY
LKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYK
HDTKLLILALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKR
HLLTQRAFKEVGIEFMDLYSHLVPVYDVEPLEKITDAYLDQYLWYEADKR
RLFPPWIKPADTEPPPLLVYKWCQGINNLQDVWETSEGECNVMLESRFEK
MYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGIIRG
LQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDIATEAAH
PIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEHPDPNNENIVGYNNKKC
WPRDARMRLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNP
NLLFNMCGFECRILPKCRTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVD
DESMQRFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREAVVNTQE
LLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPKELGGLGMLSMGHV
LIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFIDSQ
RVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYD
KGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEG
ILEHTLFKGTYFPTWEGLFWEKWKKLTNAQRSGLNQIPNRRFTLWWSPTI
NRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESIVMD
LCQVFDQELDALEIETVQKETIHPRKSYKMNSSCADILLFASYKWNVSRP
SLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYARAKFLDYTTDNM
SIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPALYV
LRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHK
TFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTA
EEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQLP
FQACLKVEKFGDLILKATEPQMVLFNLYDDWLKTISSYTAFSRLILILRA
LHVNNDRAKVILKPDKTTITEPHHIWPTLTDEEWIKVEVQLKDLILADYG
KKNNVNVASLTQSEIRDIILGMEFSSKTEWRVRAISAANLHLRTNHIYVS
SDDIKETGYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIR
CIVMVPQWGTHQTVHLPGQLPQHEYLKEMEPLGWIHTQPNESPQLSPQDV
TTHAKIMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQNTD
KGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSWNYNFMGVRHDPNMK
YELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADREDLYA
Ligand information
>8i0t Chain B (length=98) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
gguuucucuucagaucgcauaaaucuuucgccuuuuacuaaagauuuccg
uggagaggaacaaccccaauuuuuugaggccuugcuuuggcaaggcua
.<<<<<<<<<<<........<<<<<<<<...........>>>>>>>>...
>>>>>>>>>>>................<<<<<<<<....>>>>>>>>.
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB8i0t Molecular basis for the activation of human spliceosome
Resolution3.0 Å
Binding residue
(original residue number in PDB)		Q57 I132 W134 N221 G222 S223 T224 K267 E280 R409 R417 R419 R420 D423 P425 K428 N457 H461 P464 Q467 K469 R470 R474 K595 Y596 K597 R635 F639 R642 G643 V767 D768 K1294 R1298
Binding residue
(residue number reindexed from 1)		Q31 I106 W108 N195 G196 S197 T198 K241 E254 R376 R384 R386 R387 D390 P392 K395 N424 H428 P431 Q434 K436 R437 R441 K562 Y563 K564 R602 F606 R609 G610 V734 D735 K1261 R1265
Gene Ontology
Molecular Function
GO:0003723 RNA binding
GO:0005515 protein binding
GO:0008233 peptidase activity
GO:0017070 U6 snRNA binding
GO:0030619 U1 snRNA binding
GO:0030620 U2 snRNA binding
GO:0030623 U5 snRNA binding
GO:0070530 K63-linked polyubiquitin modification-dependent protein binding
GO:0097157 pre-mRNA intronic binding
GO:0140492 metal-dependent deubiquitinase activity
Biological Process
GO:0000244 spliceosomal tri-snRNP complex assembly
GO:0000375 RNA splicing, via transesterification reactions
GO:0000398 mRNA splicing, via spliceosome
GO:0006397 mRNA processing
GO:0008380 RNA splicing
GO:0071222 cellular response to lipopolysaccharide
GO:0071356 cellular response to tumor necrosis factor
Cellular Component
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005681 spliceosomal complex
GO:0005682 U5 snRNP
GO:0016020 membrane
GO:0016607 nuclear speck
GO:0030532 small nuclear ribonucleoprotein complex
GO:0046540 U4/U6 x U5 tri-snRNP complex
GO:0071005 U2-type precatalytic spliceosome
GO:0071006 U2-type catalytic step 1 spliceosome
GO:0071007 U2-type catalytic step 2 spliceosome
GO:0071013 catalytic step 2 spliceosome

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8i0t, PDBe:8i0t, PDBj:8i0t
PDBsum8i0t
PubMed39068178
UniProtQ6P2Q9|PRP8_HUMAN Pre-mRNA-processing-splicing factor 8 (Gene Name=PRPF8)

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