Structure of PDB 8exa Chain A Binding Site BS01

Receptor Information

>8exa Chain A (length=341) Species: 243230 (Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539)

IRNKAFVVRLYPNAAQTELINRTLGSARFVYNHFLARRIAAYKESGKGLT
YGQTSSELTLLKQAEETSWLSEVDKFALQNSLKNLETAYKNFFRTVKQSG
KKVGFPRFRKKRTGESYRTQFTNNNIQIGEGRLKLPKLGWVKTKGQQDIQ
GKILNVTVRRIHEGHYEASVLCEVEIPYLPAAPKFAAGVDVGIKDFAIVT
DGVRFKHEQNPKYYRSTLKRLRKAQQTLSRRKKGSARYGKAKTKLARIHK
RIVNKRQDFLHKLTTSLVREYEIIGTEHLKPDNMRKNRRLALSISDAGWG
EFIRQLEYKAAWYGRLVSKVSPDRDENAALNIRREALVAAG

Ligand information

>8exa Chain B (length=101)

gguggcugcgggaaucucagacaccuuaaacgcucauggaggcuaugguc
ugcgaagugagaaucacgcgaucgugugagguucaagagucccuuggcgc
c

<<<<.<<<...((((..>>>.>>>>.......<<<<<...<<<<..>>>>
......>>>>>..<<<<<<<..>>>>>>>.))))................
.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8exa TnpB structure reveals minimal functional core of Cas12 nuclease family.
• Resolution: 3.14 Å
• Binding residue (original residue number in PDB): K5 A6 Y12 N85 G105 P107 R108 R110 S117 G146 Q148 T158 R160 S170 Y215 R223 Q226 Q227 S230 R231 R232 K233 S236 A237 R238 K241 R252 K256 D259 K263 T266 R270 D283 R286 R289 A292 L293 Y309 K310 W313
• Binding residue (residue number reindexed from 1): K4 A5 Y11 N84 G104 P106 R107 R109 S116 G145 Q147 T157 R159 S169 Y214 R222 Q225 Q226 S229 R230 R231 K232 S235 A236 R237 K240 R251 K255 D258 K262 T265 R269 D282 R285 R288 A291 L292 Y308 K309 W312

Enzymatic activity

• Enzyme Commision number: 3.1.21.-

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003723 RNA binding
• GO:0004519 endonuclease activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006310 DNA recombination
• GO:0032196 transposition

External links

• PDB: RCSB:8exa, PDBe:8exa, PDBj:8exa
• PDBsum: 8exa
• PubMed: 37020015
• UniProt: Q7DF80|DRA2B_DEIRA RNA-guided DNA endonuclease TnpB (Gene Name=tnpB)