Structure of PDB 8dwe Chain A Binding Site BS01

Receptor Information

>8dwe Chain A (length=355) Species: 1422 (Geobacillus stearothermophilus)

TERFPAREFQRDLLDWFARERRDLPWRKDRDPYKVWVSQVMLQQTRVETV
IPYFEQFIDRFPTLEALADADEDEVLKAWEGLGYYSRVRNLHAAVKEVKT
RYGGKVPDDPDEFSRLKGVGPYTVGAVLSLAYGVPEPAVDGNVMRVLSRL
FLVTDDIAKPSTRKRFEQIVREIMAYENPGAFNEALIELGALVCTPRRPS
CLLCPVQAYCQAFAEGVAEELPVKMKKTAVKQVPLAVAVLADDEGRVLIR
KRDSTGLLANLWEFPSCETDGADGKEKLEQMVGEQYGLQVELTEPIVSFE
HAFSHLVWQLTVFPGRLVHGGPVEEPYRLAPEDELKAYAFPVSHQRVWRE
YKEWA

Ligand information

>8dwe Chain B (length=11)

aagacgtggac

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8dwe Structural Basis for Base Engagement and Stereochemistry Revealed by Alteration of Catalytic Residue Glu43 in DNA Repair Glycosylase MutY
• Resolution: 2.2 Å
• Binding residue (original residue number in PDB): Q48 R50 T53 G85 G87 Y88 Y89 R202 G260 L261 L262 H305 A306 F307 S308 H309 V346
• Binding residue (residue number reindexed from 1): Q44 R46 T49 G81 G83 Y84 Y85 R198 G256 L257 L258 H301 A302 F303 S304 H305 V342

Enzymatic activity

• Enzyme Commision number: 3.2.2.31: adenine glycosylase.

Gene Ontology

Molecular Function

• GO:0000701 purine-specific mismatch base pair DNA N-glycosylase activity
• GO:0003677 DNA binding
• GO:0003824 catalytic activity
• GO:0016798 hydrolase activity, acting on glycosyl bonds
• GO:0019104 DNA N-glycosylase activity
• GO:0032357 oxidized purine DNA binding
• GO:0034039 8-oxo-7,8-dihydroguanine DNA N-glycosylase activity
• GO:0035485 adenine/guanine mispair binding
• GO:0046872 metal ion binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:0006281 DNA repair
• GO:0006284 base-excision repair
• GO:0006298 mismatch repair
• GO:0006950 response to stress

External links

• PDB: RCSB:8dwe, PDBe:8dwe, PDBj:8dwe
• PDBsum: 8dwe
• UniProt: P83847|MUTY_GEOSE Adenine DNA glycosylase (Gene Name=mutY)