Structure of PDB 8b9k Chain A Binding Site BS01

Receptor Information
>8b9k Chain A (length=951) Species: 7227 (Drosophila melanogaster) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
IHGNWTIENAKERLNIYKQTNNIRDDYKYTPVGPEHARSFLAELSIYVPA
LNRTVTARESGSNKKSASKSCALSLVRQLFHLNVIEPFSGTLKKKKDEQL
KPYPVKLSPNLINKIDEVIKGLDLPVVNPESSVIPWAPPQANWNTWHACN
IDEGELATTSIDDLSMDYERSLRDRRQNDNEYRQFLEFREKLPIAAMRSE
ILTAINDNPVVIIRGNTGCGKTTQIAQYILDDYICSGQGGYANIYVTQPR
RISAISVAERVARERCEQLGDTVGYSVRFESVFPRPYGAILFCTVGVLLR
KLEAGLRGVSHIIVDEIHERDVNSDFLLVILRDMVDTYPDLHVILMSATI
DTTKFSKYFGICPVLEVPGRAFPVQQFFLEDIIQMTDFVPSAESEDKDEA
EINYNKVCEDKYSQKTRNAMAMLSESDVSFELLEALLMHIKSKNIPGAIL
VFLPGWNLIFALMKFLQNTNIFGDTSQYQILPCHSQIPRDEQRKVFEPVP
EGVTKIILSTNIAETSITIDDIVFVIDICKARMKLFTSHNNLTSYATVWA
SKTNLEQRKGRAGRVRPGFCFTLCSRARFQALEDNLTPEMFRTPLHEMAL
TIKLLRLGSIHHFLSKALEPPPVDAVIEAEVLLREMRCLDANDELTPLGR
LLARLPIEPRLGKMMVLGAVFGCADLMAIMASYSSTFSEVFSLDIGQRRL
ANHQKALSGTKCSDHVAMIVASQMWRREKQRGEHMEARFCDWKGLQMSTM
NVIWDAKQQLLDLLQQAGFPEECMISHEVDERIDGDDPVLDVSLALLCLG
LYPNICVHKEKRKVLTTESKAALLHKTSVNCSNLAVTFPYPFFVFGEKIR
TRAVSCKQLSMVSPLQVILFGSRKIDLAANNIVRVDNWLNFDIEPELAAK
IGALKPALEDLITVACDNPSDILRLEEPYAQLVKVVKDLCVKSAGDFGLQ
R
Ligand information
>8b9k Chain B (length=40) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
guguaaaauguugcuagcaugcuaguaacguuuuacgccc
.<<<<<<<<<<<<<<<<<..>>>>>>>>>>>>>>>>>...
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB8b9k Cryo-EM structure of MLE in complex with ADP:AlF4 and SL7modUUC RNA
Resolution4.04 Å
Binding residue
(original residue number in PDB)		K171 E195 F200 N223 K224 K225 K229 T251 K253 K255 L1030
Binding residue
(residue number reindexed from 1)		K11 E35 F40 N63 K64 K65 K69 T91 K93 K95 L823
Enzymatic activity
Enzyme Commision number 3.6.4.13: RNA helicase.
Gene Ontology
Molecular Function
GO:0001069 regulatory region RNA binding
GO:0003676 nucleic acid binding
GO:0003678 DNA helicase activity
GO:0003682 chromatin binding
GO:0003690 double-stranded DNA binding
GO:0003723 RNA binding
GO:0003724 RNA helicase activity
GO:0003725 double-stranded RNA binding
GO:0004386 helicase activity
GO:0005524 ATP binding
GO:0016787 hydrolase activity
GO:0016887 ATP hydrolysis activity
GO:0033679 3'-5' DNA/RNA helicase activity
GO:0034458 3'-5' RNA helicase activity
GO:0043138 3'-5' DNA helicase activity
GO:0106222 lncRNA binding
Biological Process
GO:0007549 sex-chromosome dosage compensation
GO:0008340 determination of adult lifespan
GO:0009047 dosage compensation by hyperactivation of X chromosome
GO:0010468 regulation of gene expression
GO:0031453 positive regulation of heterochromatin formation
GO:0032508 DNA duplex unwinding
GO:0042714 dosage compensation complex assembly
GO:0045433 male courtship behavior, veined wing generated song production
GO:0045944 positive regulation of transcription by RNA polymerase II
GO:0048675 axon extension
GO:0050684 regulation of mRNA processing
GO:2000373 positive regulation of DNA topoisomerase (ATP-hydrolyzing) activity
GO:2000765 regulation of cytoplasmic translation
Cellular Component
GO:0000228 nuclear chromosome
GO:0000785 chromatin
GO:0000805 X chromosome
GO:0005634 nucleus
GO:0005694 chromosome
GO:0005730 nucleolus
GO:0005829 cytosol
GO:0016456 X chromosome located dosage compensation complex, transcription activating
GO:0072487 MSL complex
GO:1990904 ribonucleoprotein complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8b9k, PDBe:8b9k, PDBj:8b9k
PDBsum8b9k
PubMed37989319
UniProtP24785|MLE_DROME Dosage compensation regulator mle (Gene Name=mle)

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