Structure of PDB 8b9g Chain A Binding Site BS01

Receptor Information
>8b9g Chain A (length=953) Species: 7227 (Drosophila melanogaster) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
EQRDMNEAEAFDVNAAIHGNWTIENAKERLNIYKQTNNIRDDYKYTPVGP
EHARSFLAELSIYVPALNRTVTARESGSNKKSASKSCALSLVRQLFHLNV
IEPFSGTLEQLKPYPVKLSPNLINKIDEVIKGLDLPVVNSSVIPWAPPQA
NWNTWHACNIDEGELATTSIDDLSMDYERSLRDRRQNDNEYRQFLEFREK
LPIAAMRSEILTAINDNPVVIIRGNTGCGKTTQIAQYILDDYICSGQGGY
ANIYVTQPRRISAISVAERVARERCEQLGDTVGYSVRFESVFPRPYGAIL
FCTVGVLLRKLEAGLRGVSHIIVDEIHERDVNSDFLLVILRDMVDTYPDL
HVILMSATIDTTKFSKYFGICPVLEVPGRAFPVQQFFLEDIIQMTDFVPS
AESINYNKVCEDKYSQKTRNAMAMLSESDVSFELLEALLMHIKSKNIPGA
ILVFLPGWNLIFALMKFLQNTNIFGDTSQYQILPCHSQIPRDEQRKVFEP
VPEGVTKIILSTNIAETSITIDDIVFVIDICKARMKLFTSHNNLTSYATV
WASKTNLEQRKGRAGRVRPGFCFTLCSRARFQALEDNLTPEMFRTPLHEM
ALTIKLLRLGSIHHFLSKALEPPPVDAVIEAEVLLREMRCLDANDELTPL
GRLLARLPIEPRLGKMMVLGAVFGCADLMAIMASYSSTFSEVFSLDIGQR
RLANHQKALSGTKCSDHVAMIVASQMWRREKQRGEHMEARFCDWKGLQMS
TMNVIWDAKQQLLDLLQQAGFPEECMISHEVDERIDGDDPVLDVSLALLC
LGLYPNICVHKEKRKVLTTESKAALLHKTSVNCSNLAVTFPYPFFVFGEK
IRTRAVSCKQLSMVSPLQVILFGSRKIDLAANNIVRVDNWLNFDIEPELA
AKIGALKPALEDLITVACDNPSDILRLEEPYAQLVKVVKDLCVKSAGDFG
LQR
Ligand information
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB8b9g Cryo-EM structure of MLE in complex with ADP:AlF4 and U10 RNA
Resolution2.86 Å
Binding residue
(original residue number in PDB)		A197 R442 R443 R470 G488 V489 R492 K493 V635 S636 F637 G662 W663 N664 H691 S692 Q693 T717 N718 I719 R739 Y752 P863 F894 E896 L900 Q953 Q966 H1032 T1034 T1058
Binding residue
(residue number reindexed from 1)		A53 R259 R260 R287 G305 V306 R309 K310 V430 S431 F432 G457 W458 N459 H486 S487 Q488 T512 N513 I514 R534 Y547 P658 F689 E691 L695 Q748 Q761 H827 T829 T853
Enzymatic activity
Enzyme Commision number 3.6.4.13: RNA helicase.
Gene Ontology
Molecular Function
GO:0001069 regulatory region RNA binding
GO:0003676 nucleic acid binding
GO:0003678 DNA helicase activity
GO:0003682 chromatin binding
GO:0003690 double-stranded DNA binding
GO:0003723 RNA binding
GO:0003724 RNA helicase activity
GO:0003725 double-stranded RNA binding
GO:0004386 helicase activity
GO:0005524 ATP binding
GO:0016787 hydrolase activity
GO:0016887 ATP hydrolysis activity
GO:0033679 3'-5' DNA/RNA helicase activity
GO:0034458 3'-5' RNA helicase activity
GO:0043138 3'-5' DNA helicase activity
GO:0106222 lncRNA binding
Biological Process
GO:0007549 sex-chromosome dosage compensation
GO:0008340 determination of adult lifespan
GO:0009047 dosage compensation by hyperactivation of X chromosome
GO:0010468 regulation of gene expression
GO:0031453 positive regulation of heterochromatin formation
GO:0032508 DNA duplex unwinding
GO:0042714 dosage compensation complex assembly
GO:0045433 male courtship behavior, veined wing generated song production
GO:0045944 positive regulation of transcription by RNA polymerase II
GO:0048675 axon extension
GO:0050684 regulation of mRNA processing
GO:2000373 positive regulation of DNA topoisomerase (ATP-hydrolyzing) activity
GO:2000765 regulation of cytoplasmic translation
Cellular Component
GO:0000228 nuclear chromosome
GO:0000785 chromatin
GO:0000805 X chromosome
GO:0005634 nucleus
GO:0005694 chromosome
GO:0005730 nucleolus
GO:0005829 cytosol
GO:0016456 X chromosome located dosage compensation complex, transcription activating
GO:0072487 MSL complex
GO:1990904 ribonucleoprotein complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8b9g, PDBe:8b9g, PDBj:8b9g
PDBsum8b9g
PubMed37989319
UniProtP24785|MLE_DROME Dosage compensation regulator mle (Gene Name=mle)

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