Structure of PDB 8b1n Chain A Binding Site BS01

Receptor Information

>8b1n Chain A (length=433) Species: 768670 (Calditerrivibrio nitroreducens DSM 19672)

GPHMKRYNVITIFPEMINEIFKYGVLSKGIDIGLFRVNPINLRDYTEDKH
KTVDDYQYGGGHGLVMKPEPIYKAIADLKSKKDTHVVFLDPRGEQFTQKT
AERLYNYDDITFVCGRYEGIDDRVRELMADEMISIGDFVITGGELAAVTI
IDAVARLIPGVLGDENSPNEESFTTGLLEYPHFTRPAEFMGKKVPEVLIS
GNHEEIRRWRLTESIKTTLQNRPDMILRKSLSREEEQILWSLTRGVQRKY
NIYVALMHYPMRDKEGKVVTTSITNMDLHDISRSCRTFGVKNYFVVNPMP
AQREIASRVVRHWIKGYGATYNENRKEAFEYTIITDSLASVIKSIEEKES
GSPIIIATTARYQQKAISIEKLKEIADRPILLLFGTGWGFVDDILEFADY
VLKPIHGVGDFNHLSVRSAVAIYLDRINRSFQE

Ligand information

• Ligand ID: MG
• InChI: InChI=1S/Mg/q+2
• InChIKey: JLVVSXFLKOJNIY-UHFFFAOYSA-N
• SMILES:
  ACDLabs 10.04
  OpenEye OEToolkits 1.5.0: [Mg+2]
  CACTVS 3.341: [Mg++]
• Formula: Mg
• Name: MAGNESIUM ION
• DrugBank: DB01378
• PDB chain: 8b1n Chain A Residue 501

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8b1n Are there double knots in proteins? Prediction and in vitro verification based on TrmD-Tm1570 fusion from C. nitroreducens. To be published
• Resolution: 2.0 Å
• Binding residue (original residue number in PDB): E167 E168 E176
• Binding residue (residue number reindexed from 1): E170 E171 E179
• Annotation score: 1

Enzymatic activity

• Enzyme Commision number: 2.1.1.228: tRNA (guanine(37)-N(1))-methyltransferase.

Gene Ontology

Molecular Function

• GO:0008168 methyltransferase activity
• GO:0046872 metal ion binding
• GO:0052906 tRNA (guanine(37)-N1)-methyltransferase activity

Biological Process

• GO:0002939 tRNA N1-guanine methylation
• GO:0006400 tRNA modification
• GO:0008033 tRNA processing
• GO:0032259 methylation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:8b1n, PDBe:8b1n, PDBj:8b1n
• PDBsum: 8b1n
• PubMed: 38903539
• UniProt: E4THH1