Structure of PDB 7zs5 Chain A Binding Site BS01

Receptor Information

>7zs5 Chain A (length=224) Species: 4932 (Saccharomyces cerevisiae)

MATRTQFENSNEIGVFSKLTNTYCLVAVGGSENFYSAFEAELGDAIPIVH
TTIAGTRIIGRMTAGNRRGLLVPTQTTDQELQHLRNSLPDSVKIQRVEER
LSALGNVICCNDYVALVHPDIDRETEELISDVLGVEVFRQTISGNILVGS
YCSLSNQGGLVHPQTSVQDQEELSSLLQVPLVAGTVNRGSSVVGAGMVVN
DYLAVTGLDTTAPELSVIESIFRL

Ligand information

>7zs5 Chain 1 (length=3248)

uugaccucaaaucagguaggaguacccgcugaacuuaagcauaucaauaa
gcggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaagc
ggcaaaagcucaaauuugaaaucugguaccuucggugcccgaguuguaau
uuggagagggcaacuuuggggccguuccuugucuauguuccuuggaacag
gacgucauagagggugagaaucccguguggcgaggagugcgguucuuugu
aaagugccuucgaagagucgaguuguuugggaaugcagcucuaagugggu
gguaaauuccaucuaaagcuaaauauuggcgagagaccgauagcgaacaa
guacagugauggaaagaugaaaagaacuuugaaaagagagugaaaaagua
cgugaaauuguugaaagggaagggcauuugaucagacaugguguuuugua
aucucgcauuucacugggccagcaucaguuuugguggcaggauaaaucca
uaggaauguagcuugccucgguaaguauuauagccugugggaauacugcc
agcugggacugaggacugcgacguaagucaaggaugcuggcauaaugguu
auaugccgcccgucuugaaacacggaccaaggagucuaacgucuaugcga
guguuuggguguaaaacccauacgcguaaugaaagugaacguagguuggg
gccucgcaagaggugcacaaucgaccgauccugaugucuucggauggauu
ugaguaagagcauagcuguugggacccgaaagauggugaacuaugccuga
auagggugaagccagaggaaacucugguggaggcucguagcgguucugac
gugcaaaucgaucgucgaauuuggguauaggggcgaaagacuaaucgaac
caucuaguagcugguuccugccgaaguuucccucaggauagcagaagcuc
guaucaguuuuaugagguaaagcgaaugauuagagguuccggggucgaaa
ugaccuugaccuauucucaaacuuuaaauauguaagaaguccuuguuacu
uaauugaacguggacauuugaaugaagagcuuuuagugggccauuuuugg
uaagcagaacuggcgaugcgggaugaaccgaacguagaguuaaggugccg
gaauacacgcucaucagacaccacaaaagguguuaguucaucuagacagc
cggacgguggccauggaagucggaauccgcuaaggaguguguaacaacuc
accggccgaaugaacuagcccugaaaauggauggcgcucaagcguguuac
cuauacucuaccgucaggguugauaugaugcccugacgaguaggcaggcg
uggaggucagugacgaagccuagaccguaaggucgggucgaacggccucu
agugcagaucuuggugguaguagcaaauauucaaaugagaacuuugaaga
cugaaguggggaaagguuccacgucaacagcaguuggacguggguuaguc
gauccuaagagauggggaagcuccguuucaaaggccugauuuuaugcagg
ccaccaucgaaagggaauccgguuaagauuccggaaccuggauauggauu
cuucacgguaacguaacugaauguggagacgucggcgcgagcccugggag
gaguuaucuuuucuucuuaacagcuuaucaccccggaauugguuuauccg
gagauggggucuuauggcuggaagaggccagcaccuuugcuggcuccggu
gcgcuugugacggcccgugaaaauccacaggaaggaauaguuuucaugcc
aggucguacugauaaccgcagcaggucuccaaggugaacagccucuaguu
gauagaauaauguagauaagggaagucggcaaaauagauccguaacuucg
ggauaaggauuggcucuaagggucggguagugagggccuuggucagacgc
uuguuguagacggccuuggucuugcuacaauuaacgaucaacuuagaacu
gguacggacaaggggaaucugacugucuaauuaaaacauagcauugcgau
ggucagaaagugauguugacgcaaugugauuucugcccagugcucugaau
gucaaagugaagaaauucaaccaagcgcggguaaacggcgggaguaacua
ugacucucuuaagguagccaaaugccucgucaucuaauuagugacgcgca
ugaauggauuaacgagauucccacugucccuaucuacuaucuagcgaaac
cacagccaagggaacgggcuuggcagaaucagcggggaaagaagacccug
uugagcuugacucuaguuugacauugugaagagacauagagggcuaccuu
uauaguuucuuuacuuauucaaugaagcggagcuggaauucauuuuccac
guucuagcauucaaggucccauucggggcugauccggguugaagacauug
ucagguggggaguuuggcuggggcggcacaucuguuaaacgauaacgcag
auguccuaaggggggcucauggagaacagaaaucuccaguagaacaaaag
gguaaaagcccccuugauuuugauuuucagugugaauacaaaccaugaaa
guguggccuaucgauccuuuagucccucggaauuugaggcuagaggugcc
agaaaaguuaccacagggauaacuggcuuguggcagucaagcguucauag
cgacauugcuuuuugauucuucgaugucggcucuuccuaucauaccgaag
cagaauucgguaagcguuggauuguucacccacuaauagggaacgugagc
uggguuuagaccgucgugagacagguuaguuuuacccuacugaugaaugu
uaccgcaauaguaauugaacuuaguacgagaggaacaguucauucggaua
auugguuuuugcggcugucugaucaggcauugccgcgaagcuaccauccg
cuggauuauggcugaacgccucuaagucagaauccaugcuagaacgcggu
gauuucuuugcuccacacaauauagauggauacgaauaaggcguccuugu
ggcgucgcugaaccauagcaggcuagcaacggugcacuuggcggaaaggc
cuugggugcuugcuggcgaauugcaaugucauuuugcguggggauaaauc
auuuguauacgacuuagauguacaacgggguauuguaagcaguagaguag
ccuuguuguuacgaucugcugagauuaagccuuuguugucugauuugu

........................<<<<<<.....<<....>>......>
>>>>.>...<.....<<....>>..<<<<<......<<.....>>.....
>>>>>..<<<...........<<.<<<<<<...>>>>>>.>>........
.....<<<<<<.<<<<<<<<<<<<<<<<<<<<<<....<<<<......>>
>>.((......<<<.....))>>>..>.>>>>>>>>>..>>>>>>>....
>>>>>>>>>>>........<<<<<<<........>>>>>>>.....<<<<
<..((((((>>>>>..>>>....>..........................
...<<....>>...............<<<<....>>>>............
......................<<<<<.<<<<<......<<<....<<..
......>>....>>>..<<<<<<<<<<<<<<<<<<<<<<<.....<<<<<
<<<<......<<<<<<....>>>>>>.......>>>>>>>>..>.>>>>>
.>>>>>>>>>>........<<<....>>>....>>>>>>>>.....>>>>
>.>>>>>..<<<<........>>>><<<<<<....<<<<<<.<<<<<<..
<<<...<<<<.....>>>>...>>>...<<....>>.......<<<<<..
<<<<<....>>>>>...>>>>>.<<<<<<<<...<......>...>>>.>
>>.>>....>>>>>>.>>>>>>..<<.<..<<<<<<<.....<<<<<<<<
<..<<<....<<<<<<<....>>>>>>>.....>>>...<<<..<<.<<.
.......>>>>.>>>....>>>>>>>>>...<<.......>>......>>
>>>>...>..>.>>..<<<<<<....<.<<<....>>>.><<<<<<<<<<
...<<<.<<<<<<<.....<<<....<<<....<<<<<..<<<<<<....
.>>>>>>>>>>>....>>>..>.>>.....>>>>>...<<<<.<<<<.<.
.....>>>>>.>>>>.....>>>>>.>>>>>>>>.>>..<<<<.<<<<<.
....>>>>>.>>>>....<<<......>>>...<<<<<<<..<<<<(((.
....<<<<<<.....<<..........<<<.<<<<<<<<<........<<
<<<.<<<...<<.........>>...>>>.........<<......>>..
.>>>>>....>>>>>>>>>>>>..........))).>>...>>>>>>.>>
>>..>>>>>>><<<<<<<<<.........>>>>>>>>>....>>>>>>..
<<<<<<<<.<<......<<<<.<<<<....>>>>>>>>...>>>>>>>>>
>........>>>>>><<.....<<.....<<<<<<.......>>>>>><<
<<.....<<<<<.<<...<<<<<<...<<.....>>.>>>>>>....((<
<<<<<...<<....<<<....>>>........<<.<<<........>.>>
>>....>>....>>>..<<<<<........>>>>>))<<<<.<<<<<<<.
....<<<....>>>...<.{{<<<<<<...<<<<.<<<<<<<<<<<<<<<
<<<..[[[..>>>>>><...<<<<<....<<<<<<....<<<.....>>>
....>>>>>>.....>>>>>....><<<<<<<<....>>>>>>>>>>>>.
>.>>>>>>>.>>>>.........>>>>>>...>......>>>..>>>>>>
>>.>>>..>>.....<<......>>>>>>>.<<<<.....>>>>..>>>>
..<<<<.....<<<<<<<.<<<........<.....<<<<<....<<..>
>.....>>>>><<.<<<<<<<<<<<.<<<<<<........<<<.....<.
......>.....>>>.......>>>>>>......>>>>>..>>>>>>.>>
............<<<<<<<<<..<<<.........>>>..<<<<<<<...
.<<<<.....>>>>......>>>>>>>...<<..<<<..<<<<<<.....
<<.....<<<<....>>>>.....>>..>>>>..>>>>><<<<<<.....
..>>>>>>........<...........<<<<<.......>>>>>.....
>.....>>....>>.>>>>>>>..>.>>>.>>>>>>>..>>>>>>....>
>....<<<<<........>>>>>....<<<<<..<<<.<<......<<<<
.<<<<<<..<<<<<.<<<<<<<<..<<.<<<<<<<<<<<<<......>>>
>>>.>>>>>>>.>>...<<<<<.....<<<<..<<<<<......>>>>>.
.........}}...<<<<<<....>>>>>>..>>>>..>>>>>.....>>
>>>>>>..>>>>>...<<<.....>>>..<<<<<<...........>>>>
>>..<<...<<<<<<<...<<<<<<.......>>>>>>.....<<...((
(>>....>>>>>>>...<<<.<<<<<.......>>>>.>>>>.<<<....
>>>.>>)))......<<<<<<<..<<<<.))))))>>>>>>>>>>>....
.....>>>>..>>>>>>.......<<.<.<.<<<<<<...<<.......>
>...>>>>>>...>>.>.>.......<<<<<.<<<<<<..<.<<<<<<<.
.....>>>>>>>...>.<<<.........>>>.....>>>>>..>...>>
>.>>....<<<<<<<....>>>.>>>>....>>..>>>.>>>>>....<<
<<<<<<..<<<<.<.<<<<<<...............>>>>>>.><<<<<.
..<<<<<...<<<<<<<<<<]]].>>>>>..>>>>>..>>>>>..>>>>>
.<<<<...<<<<<...........>>>>>...>>>>.>>>>....>>>>>
>>>.......<<<<<<.<<<.<<....<<..........<<<<<<.....
>>>>>>......>>.......<<<<<<...<<<<<.<..<<<......>>
>..>.>>>>>.>>>>>>.............>>.>>>.>>>>>>..<<<<<
.<.............>.....<<<<<<<<<.<<<....<<<<.<..<<<<
<......>>>>>..>>>>>...>>>....>>>>.>>>>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7zs5 Structural basis for clearing of ribosome collisions by the RQT complex.
• Resolution: 3.2 Å
• Binding residue (original residue number in PDB): E8 N9 S31
• Binding residue (residue number reindexed from 1): E8 N9 S31

Gene Ontology

Molecular Function

• GO:0003743 translation initiation factor activity
• GO:0005515 protein binding
• GO:0043022 ribosome binding
• GO:0043023 ribosomal large subunit binding

Biological Process

• GO:0000054 ribosomal subunit export from nucleus
• GO:0000460 maturation of 5.8S rRNA
• GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0000466 maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0000470 maturation of LSU-rRNA
• GO:0006364 rRNA processing
• GO:0006412 translation
• GO:0006413 translational initiation
• GO:0042254 ribosome biogenesis
• GO:0042256 cytosolic ribosome assembly
• GO:0042273 ribosomal large subunit biogenesis
• GO:1902626 assembly of large subunit precursor of preribosome

Cellular Component

• GO:0005634 nucleus
• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0030687 preribosome, large subunit precursor

External links

• PDB: RCSB:7zs5, PDBe:7zs5, PDBj:7zs5
• PDBsum: 7zs5
• PubMed: 36801861
• UniProt: Q12522|IF6_YEAST Eukaryotic translation initiation factor 6 (Gene Name=TIF6)