Structure of PDB 7wju Chain A Binding Site BS01

Receptor Information
>7wju Chain A (length=407) Species: 1765684 (Sulfoacidibacillus thermotolerans) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
MIKVYRYEIVKPLDLDWKEFGTILRQLQQETRFALNKATQLAWEWMGFSS
DYKDNHGEYPKSKDILGYTNVHGYAYHTIKTKAYRLNSGNLSQTIKRATD
RFKAYQKEILRGDMSIPSYKRDIPLDLIKENISVNRMNHGDYIASLSLLS
NPAKQEMNVKRKISVIIIVRGAGKTIMDRILSGEYQVSASQIIHDDRKNK
WYLNISYDFEPQTRVLDLNKIMGIALGVAVAVYMAFQHTPARYKLEGGEI
ENFRRQVESRRISMGGHGRDKRIKPIEQLRDKIANFRDTTNHRYSRYIVD
MAIKEGCGTIQMEDLTNIRDIGSRFLQNWTYYDLQQKIIYKAEEAGIKVI
KIDPQYTSQRCSECGNIDSGNRIGQAIFKCRACGYEANADYNAARNIAIP
NIDKIIA
Ligand information
>7wju Chain C (length=133) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
ucgucgguucagcgacgauaagccgagaagugccaauaaaacgugguuug
guaacgcucgguaagguagcgcugaaacuccgugcacaaagaccgcacgg
acgcuugagugugaaccuggcguuuuuccauag
<<<<<<.((((.>>>>>>...<<<<<<..<<<<<<<....<..>...>>>
>>.>>.>>>>>>..((..<..>......<<<<<<<.......))>>>>>>
><<<<..>>>>))))..................
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB7wju Structure-directed functional evolution of the miniature CRISPR-AsCas12f1 system
Resolution2.69 Å
Binding residue
(original residue number in PDB)		K3 V4 Y5 R6 E8 K11 D16 W17 R101 M114 S115 P117 S118 Y119 K120 P124 R136 H139 R170 G171 Q191 K198 K200 Y202 G277 H278 G279 R280 R283 N296 T300 H303 R304 R307 Y351
Binding residue
(residue number reindexed from 1)		K3 V4 Y5 R6 E8 K11 D16 W17 R101 M114 S115 P117 S118 Y119 K120 P124 R136 H139 R170 G171 Q191 K198 K200 Y202 G266 H267 G268 R269 R272 N285 T289 H292 R293 R296 Y340
Enzymatic activity
Enzyme Commision number 3.1.-.-
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003723 RNA binding
GO:0004519 endonuclease activity
GO:0046872 metal ion binding

View graph for
Molecular Function
External links
PDB RCSB:7wju, PDBe:7wju, PDBj:7wju
PDBsum7wju
PubMed
UniProtA0A2U3D0N8|CS12F_SULT2 CRISPR-associated endodeoxyribonuclease Cas12f1 (Gene Name=cas12f1)

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