Structure of PDB 7vw3 Chain A Binding Site BS01

Receptor Information
>7vw3 Chain A (length=1052) Species: 1280 (Staphylococcus aureus) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
KRNYILGLAIGITSVGYGIIDYETRDVIDAGVRLFKEANVENNEGRRSKR
GARRLKRRRRHRIQRVKKLLFDYNLLTDHSELSGINPYEARVKGLSQKLS
EEEFSAALLHLAKRRGVHNVNEVEEDTGNELSTKEQISRNSKALEEKYVA
ELQLERLKKDGEVRGSINRFKTSDYVKEAKQLLKVQKAYHQLDQSFIDTY
IDLLETRRTYYEGPGEGSPFGWKDIKEWYEMLMGHCTYFPEELRSVKYAY
NADLYNALNDLNNLVITRDENEKLEYYEKFQIIENVFKQKKKPTLKQIAK
EILVNEEDIKGYRVTSTGKPEFTNLKVYHDIKDITARKEIIENAELLDQI
AKILTIYQSSEDIQEELTNLNSELTQEEIEQISNLKGYTGTHNLSLKAIN
LILDELWHTNDNQIAIFNRLKLVPKKVDLSQQKEIPTTLVDDFILSPVVK
RSFIQSIKVINAIIKKYGLPNDIIIELAREKNSKDAQKMINEMQKRNRQT
NERIEEIIRTTGKENAKYLIEKIKLHDMQEGKCLYSLEAIPLEDLLNNPF
NYEVDHIIPRSVSFDNSFNNKVLVKQEEASKKGNRTPFQYLSSSDSKISY
ETFKKHILNLAKGKGRISKTKKEYLLEERDINRFSVQKDFINRNLVDTRY
ATRGLMNLLRSYFRVNNLDVKVKSINGGFTSFLRRKWKFKKERNKGYKHH
AEDALIIANADFIFKEWKKLDKAKKVMENQMFEEKQAESMPEIETEQEYK
EIFITPHQIKHIKDFKDYKYSHRVDKKPNRELINDTLYSTRKDDKGNTLI
VNNLNGLYDKDNDKLKKLINKSPEKLLMYHHDPQTYQKLKLIMEQYGDEK
NPLYKYYEETGNYLTKYSKKDNGPVIKKIKYYGNKLNAHLDITDDYPNSR
NKVVKLSLKPYRFDVYLDNGVYKFVTVKNLDVIKKENYYEVNSKCYEEAK
KLKKISNQAEFIASFYNNDLIKINGELYRVIGVNNDLLNRIEVNMIDITY
REYLENMNDKRPPRIIKTIASKTQSIKKYSTDILGNLYEVKSKKHPQIIK
KG
Ligand information
>7vw3 Chain B (length=104) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
guaccgcuccagucguucaugguuuuaguacucuggaaacagaaucuacu
aaaacaaggcaaaaugccguguuuaucucgucaacuuguuggcgagauuu
uuuu
.....................<<<<<<<<..<<<<....>>>>....>>>
>>>>>..<<<.....>>>......<<<<<<.<<<....>>>.>>>>>>..
....
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB7vw3 Full-Length Model of SaCas9-sgRNA-DNA Complex in Cleavage State.
Resolution3.8 Å
Binding residue
(original residue number in PDB)
N43 N44 R47 R48 R51 R54 R55 L56 R58 R59 R63 L83 S84 G85 I86 N87 P88 K114 R115 R116 G117 V164 R165 N169 R208 R209 T210 Y211 G214 P215 G216 P220 F221 Y239 K248 Y249 N257 R314 H393 N394 L395 Q414 P425 P448 R452 K459 K466 R654 M732 R774 K777 N780 R781 E782 L783 I784 D786 S790 L828 M829 H832 D833 K867 S869 I877 K878 K879 I880 K881 R901 L931 K935 T1032 D1033 I1034 N1037 Y1039 V1041 K1051 G1053
Binding residue
(residue number reindexed from 1)
N42 N43 R46 R47 R50 R53 R54 L55 R57 R58 R62 L82 S83 G84 I85 N86 P87 K113 R114 R115 G116 V163 R164 N168 R207 R208 T209 Y210 G213 P214 G215 P219 F220 Y238 K247 Y248 N256 R313 H392 N393 L394 Q413 P424 P447 R451 K458 K465 R653 M731 R773 K776 N779 R780 E781 L782 I783 D785 S789 L827 M828 H831 D832 K866 S868 I876 K877 K878 I879 K880 R900 L930 K934 T1031 D1032 I1033 N1036 Y1038 V1040 K1050 G1052
Enzymatic activity
Enzyme Commision number 3.1.-.-
Gene Ontology
Molecular Function
GO:0003676 nucleic acid binding
GO:0003677 DNA binding
GO:0003723 RNA binding
GO:0004519 endonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0043571 maintenance of CRISPR repeat elements
GO:0051607 defense response to virus

View graph for
Molecular Function

View graph for
Biological Process
External links
PDB RCSB:7vw3, PDBe:7vw3, PDBj:7vw3
PDBsum7vw3
PubMed36674715
UniProtJ7RUA5|CAS9_STAAU CRISPR-associated endonuclease Cas9 (Gene Name=cas9)

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