Structure of PDB 7tid Chain A Binding Site BS01

Receptor Information

>7tid Chain A (length=486) Species: 4932 (Saccharomyces cerevisiae)

VREEDKLWTVKYAPTNLQQVCGNKGSVMKLKNWLANWENSKKNSFKHAGK
DGSGVFRAAMLYGPPGIGKTTAAHLVAQELGYDILEQNASDVRSKTLLNA
GVKNALDNMSVVGYFKHNEEAQNLNGKHFVIIMDEVDGMSGGDRGGVGQL
AQFCRKTSTPLILICNERNLPKMRPFDRVCLDIQFRRPDANSIKSRLMTI
AIREKFKLDPNVIDRLIQTTRGDIRQVINLLSTISTTTKTINHENINEIS
KAWEKNIALKPFDIAHKMLDGQIYSDIGSRNFTLNDKIALYFDDFDFTPL
MIQENYLSTRPSVLKPGQSHLEAVAEAANCISLGDIVEKKIRSSEQLWSL
LPLHAVLSSVYPASKVAGHMAGRINFTAWLGQNSKSAKYYRLLQEIHYHT
RLGTSTDKIGLRLDYLPTFRKRLLDPFLKQGADAISSVIEVMDDYYLTKE
DWDSIMEFFVGPDVTTAIIKKIPATVKSGFTRKYNS

Ligand information

>7tid Chain I (length=24)

tttttttatgtactcgtagtgtct

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7tid Cryo-EM structures reveal high-resolution mechanism of a DNA polymerase sliding clamp loader.
• Resolution: 3.3 Å
• Binding residue (original residue number in PDB): S384 T386 R434 N459 P461 F552 D586 F587 L590 R632 S633 Q636 W638 R663 I664 F666 L670
• Binding residue (residue number reindexed from 1): S94 T96 R144 N169 P171 F262 D296 F297 L300 R342 S343 Q346 W348 R373 I374 F376 L380

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003689 DNA clamp loader activity
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0016887 ATP hydrolysis activity

Biological Process

• GO:0000278 mitotic cell cycle
• GO:0006260 DNA replication
• GO:0006261 DNA-templated DNA replication
• GO:0006271 DNA strand elongation involved in DNA replication
• GO:0006272 leading strand elongation
• GO:0006281 DNA repair
• GO:0006298 mismatch repair
• GO:0051301 cell division

Cellular Component

• GO:0005634 nucleus
• GO:0005663 DNA replication factor C complex

External links

• PDB: RCSB:7tid, PDBe:7tid, PDBj:7tid
• PDBsum: 7tid
• PubMed: 35179493
• UniProt: P38630|RFC1_YEAST Replication factor C subunit 1 (Gene Name=RFC1)