Structure of PDB 7ssg Chain A Binding Site BS01

Receptor Information

>7ssg Chain A (length=382) Species: 83333 (Escherichia coli K-12)

RAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFA
NWPVRIEMISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLG
LLIVDEEHRFGVRHKERIKAMRANVDILTLTATPIPRTLNMAMSGMRDLS
IIATPPARRLAVKTFVREYDSMVVREAILREILRGGQVYYLYNDVENIQK
AAERLAELVPEARIAIGHGQMRERELERVMNDFHHQRFNVLVCTTIIETG
IDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWLLTPHPKAMTT
DAQKRLEAIASLEDLGAGFALATHDLEIRGAG

Ligand information

>7ssg Chain B (length=29)

tttttttttttgcctcgctgccgtcgcca

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7ssg Mechanism of transcription modulation by the transcription-repair coupling factor.
• Resolution: 5.2 Å
• Binding residue (original residue number in PDB): R929 D949 L950
• Binding residue (residue number reindexed from 1): R355 D375 L376

Enzymatic activity

• Enzyme Commision number: 3.6.4.-

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0003684 damaged DNA binding
• GO:0004386 helicase activity
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0015616 DNA translocase activity
• GO:0016787 hydrolase activity
• GO:0043175 RNA polymerase core enzyme binding

Biological Process

• GO:0000716 transcription-coupled nucleotide-excision repair, DNA damage recognition
• GO:0006281 DNA repair
• GO:0006283 transcription-coupled nucleotide-excision repair
• GO:0006294 nucleotide-excision repair, preincision complex assembly
• GO:0006355 regulation of DNA-templated transcription
• GO:0006974 DNA damage response

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:1990391 DNA repair complex

External links

• PDB: RCSB:7ssg, PDBe:7ssg, PDBj:7ssg
• PDBsum: 7ssg
• PubMed: 35641110
• UniProt: P30958|MFD_ECOLI Transcription-repair-coupling factor (Gene Name=mfd)