Structure of PDB 7of1 Chain A Binding Site BS01

Receptor Information

>7of1 Chain A (length=208) Species: 559292 (Saccharomyces cerevisiae S288C)

GRVIRNQRKGAGSIFTSHTRLRQGAAKLRTLDYAERHGYIRGIVKQIVHD
SGRGAPLAKVVFRDPYKYRLREEIFIANEGVHTGQFIYAGKKASLNVGNV
LPLGSVPEGTIVSNVEEKPGDRGALARASGNYVIIIGHNPDENKTRVRLP
SGAKKVISSDARGVIGVIAGGGRVDKPLLKAGRAFHKYRLKRNSWPKTRG
VAMNPVDH

Ligand information

>7of1 Chain 1 (length=2787)

uugaccucaaaucagguaggaguacccgcugaacuuaagcauaucaauaa
gcggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaagc
ggcaaaagcucaaauuugaaaucugguaccuucggugcccgaguuguaau
uuggagagggcaacuuuggggccguuccuugucuauguuccuuggaacag
gacgucauagagggugagaaucccguguggcgaggagugcgguucuuugu
aaagugccuucgaagagucgaguuguuugggaaugcagcucuaagugggu
gguaaauuccaucuaaagcuaaauauuggcgagagaccgauagcgaacaa
guacagugauggaaagaugaaaagaacuuugaaaagagagugaaaaagua
cgugaaauuguugaaagggaagggcauuugaucagacaggccagcaucag
uuuugguggcaggauaaauccauaggaauguagcuugccucgguaaguau
uauagccugugggaauacugccagcugggacugaggacugcgacguaagu
caaggaugcuggcauaaugguuauaugccgcccgucuugaaacacggacc
aaggagucuaacgucuaugcgaguguuuggguguaaaacccauacgcgua
augaaagugaacguagguuggggccucgcaagaggugcacaaucgaccga
uccugaugucuucggauggauuugaguaagagcauagcuguugggacccg
aaagauggugaacuaugccugaauagggugaagccagaggaaacucuggu
ggaggcucguagcgguucugacgugcaaaucgaucgucgaauuuggguau
aggggcgaaagacuaaucgaaccaucuaguagcugguuccugccgaaguu
ucccucggauagcagaagcucguaucaguuuuaugaguaaauauguaaga
aguccuuguuuaauugaacguggacauuugaaugaagagcuuuuaguggg
ccauuuuugguaagaacuggcgaugcgggaugaaccgaacguagaguuaa
ggugccggaauacacgcucaucagacaccacaaaagguguuaguucaucu
agacagccggacgguggccauggaagucggaauccgcuaaggagugugua
acaacucaccggccgaaugaacuagcccugaaaauggauggcgcucaagc
guguuaccuauacucuaccgucaggguuggaugcccugacgaguaggcag
gcguggaggucagugacgaagccuagaccguaaggucgggucgaacggcc
ucuagugcagaucuuggugguaguagcaaauauucaaaugagaacuuuga
agacugaaguggggaaagguuccacgucaacagcaguuggacguggguua
gucgauccuaagagauggggaagcuccguuucaaaggccugaugcaggcc
accaucgaaagggaauccgguuaagauuccggaaccuggauauggauucu
ucacgguaacguaacugaauguggagacgucggcgcgagcccugggagga
guuaucuuuucuucuuaacagcuuaucaccccggaauugguuuauccgga
gauggggucuuauggcuggaagaggccagcaccuuugcuggcuccggugc
gcuugugacggcccgugaaaauccacaggaaggaauaguuuucaugccag
gucguacugauaaccgcagcaggucuccaaggugaacagccucuaguuga
uagaauaauguagauaagggaagucggcaaaauagauccguaacuucggg
auaaggauuggcucuaagggucggacgaucaacuuagaacuggacggaca
aggggaaucugacugucuaauuaaaacauagcauugcgauggucagaaag
ugauguugacgcaaugugauuucugcccagugcugucaaagugaagaaau
ucaaccaagcgcggguaaacggauaacgagauucccacugucccuaucua
cuaucuagcgaaaccacagccaagggaacgggcuuggcagaaucagcggg
gaaagaccugagcuugacucuaguuugacauugugaagagacauguuucu
uuacuuauucaaugaagcggagcuggaaccacguucuagcauucaagguc
ccauucggggcugauccggguugaagacaugucagguggggaguuuuagu
cccucggaauuugaggcuagaggaaaaguuaccacaggaagcuuguggca
gucaagcgacauugcuaugucggcucuuccuaucauaccgaagcagaauu
cgguaagcguuggauuguucacccacuaauagggaacgugagcuggguuu
agaccgucgugagacagguuaguacccuacugaugaauguuaccgcaaua
guaauugaacuuaguacgagaggaacaguucauucggauaauugguuuuu
gcggcugucugaucaggcauugccgcgaagcuaccauccgcuggauuaug
gcugaacgccucuaagucagaauccaugcuagaacgcggugauuucuuug
cuccacacaauauagauggauacgaauaaggcguccuuguggcgucgcug
aaccauagcaggcuagcaacggugcacuuggcggaaaggccuugggugcu
ugcuggcgaauugcaaugucauuuugcguggggauaaaucauuuguauac
gacuuagauguacaacgggguauuguaagcaguagaguagccuguuguua
cgaucugcugagauuaagccuuuguugucugauuugu

........................<<<<<<.....<......>......>
>>>>.>.........<<....>>..<<<<<......<<.....>>.....
>>>>>..[[[...........<<.<<<<<<...>>>>>>.>>........
.....<<<<<<.<<<<<<<<<<<<<<<<<<<<<<....<<<<......>>
>>.((......<<<.....))>>>..>.>>>>>>>>>..>>>>>>>....
>>>>>>>>>>>........<<<<<<<........>>>>>>>.....((((
(..<<<<<<)))))..]]]...............................
...<<....>>...............<<<<....>>>>............
......................<<<<<.<<<<<......<<<<<<<.<<<
<<<<<<<<<<<<.....<<<<<<<<<......<<<<<<....>>>>>>..
.....>>>>>>>.>.>.>>>>>.>>>>>>>>>>........<<<....>>
>.....>>>>>>>.....>>>>>.>>>>>..<<<<........>>>><<<
<<<....<<<<<<.<<<<<<..<<<...<<<<.....>>>>...>>>...
<<....>>.......<<<<<..<<<<<....>>>>>...>>>>>.<<<<.
<<<...<......>...>>>..>>.>>....>>>>>>.>>>>>>..<<.<
...<<<<<<.....<<<<<<<<<..<<<....<<<<<<<....>>>>>>>
.....>>>...<<<<.<<.<<........>>>>>>>>....>>>>>>>>>
...<<.......>>......>>>>>>......>.>>..<<<<<<....<.
<<<...>>>.><<<<<<<<<<...<<<.<<<<<<<........>>>>>..
.<<<<.<<<........>>>.>>>>.....>>>>>.>>>>>>>>.>>..<
<<<.<<<<....>>>>.>>>>....<<<......>>>...<<<<<<<..<
<<<(((.....<<<<<<.....<<..........<<<.<<<<<<<<<<..
.....<<<<<.<<<...<<.........>>...>>>.........<<...
...>>...>>>>>...>>>>>>>>>>>>>..........))).>>...>>
>>>>.>>>>..>>>>>>><<<<<<<<<.....>>>>>>>>>....>>>>>
><.<<<<<<<<.<<......<<<<.<<<<....>>>>>>>>...>>>>>>
>>>>..>.....>>>>>><<.....<<.....<<<<<<.......>>>>>
><<<<.....<<<<<.<<...<<<<<<...<<.....>>.>>>>>>....
((<<<<<<...<<....<<<....>>>.........<<<<<...>>>>>.
....>>....>>...<<<<<........>>>>>))<<<<.<<<<<<<...
..<<<....>>>...<.{{<<<<<<...<<<<.<<<<<<<.<<<<<<<<<
<..[[[..>>>>>><...<<<<<....<<<<<<....<<<.....>>>..
..>>>>>>.....>>>>>....><<<<<<<<....>>>>>>>>>>>>...
>>>>>>>.>>>>.........>>>>>>...>......>>>..>>>>>>>>
>>>>..>>.....<<......>>>>>>>.<<<<.....>>>>..>>>>..
<<<<.....<<<<<<<.<<...............<<<<<.....<..>..
....>>>>><<.<<<<<<<<<<<..>>>>>..>>>>>>.>>.........
..<<<<<<<<<..<<<.........>>>..<<<<<<<....<<<<.....
>>>>......>>>>>>>........<<..<<<<.<<.....<<<<....>
>>>.....>>....>>..>>>>....>>.>>>>>>>.....>>.>>>>>>
>..>>>>>>....>>....<<<<<........>>>>>....(((((..[[
[.........((((..<<<<<.<<<<<<<<..<<<<<<.<<<..>>>.>>
>>>>...<<<<<.....<<<<..<<<..>>>..........}}...<<<<
<<....>>>>>>..>>>>..>>>>>....>>>>>>>>..>>>>>..[[[.
.((((.>>>>>>))))]]].......))))................<<<<
<<........>>>>>>.....<<<.<<<<<<..<.<<<<<<<......>>
>>>>>...>.<<<.........>>>.....>>>>>..>...>>>......
.<<<<<<<....>>>.>>>>.....]]].)))))....<<<<<<<<..<<
<<.<.<<<<<<...............>>>>>>.><<<<<...<<<<....
<<<<<<<<<<]]].>>>>>..>>>>>...>>>>..>>>>>.<<<<...<<
<<<...........>>>>>...>>>>.>>>>....>>>>>>>>.......
<<<<<<.<<<.<<....<<..........<<<<<<.....>>>>>>....
..>>.......<<<<<<...<<<<<.<..<<<......>>>..>.>>>>>
.>>>>>>.............>>.>>>.>>>>>>..<<<<<.<..<.....
..>..>.....<<<<<.<<<.<<<....<<<<....<<<<<.....>>>>
>...>>>>...>>>....>>>..>>>>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7of1 Analysis of subunit folding contribution of three yeast large ribosomal subunit proteins required for stabilisation and processing of intermediate nuclear rRNA precursors.
• Resolution: 3.1 Å
• Binding residue (original residue number in PDB): G2 R3 V4 Q8 R9 G11 S14 I15 H19 T20 R21 R23 G25 A26 Y34 R37 H38 G39 Y40 K46 H50 S52 R54 Y67 K68 Y69 R70 L71 G85 F87 Y89 K93 A94 S95 E118 K119 A125 L126 A127 R128 A129 S130 N132 L150 P151 S152 K156 G173 R174 V175 K177 L179 L180 K181 A182 G183 A185 F186 H187 K188 R190 L191 R193 N194 W196 P197 T199 R200 A203 M204 N205 V207 D208
• Binding residue (residue number reindexed from 1): G1 R2 V3 Q7 R8 G10 S13 I14 H18 T19 R20 R22 G24 A25 Y33 R36 H37 G38 Y39 K45 H49 S51 R53 Y66 K67 Y68 R69 L70 G84 F86 Y88 K92 A93 S94 E117 K118 A124 L125 A126 R127 A128 S129 N131 L149 P150 S151 K155 G172 R173 V174 K176 L178 L179 K180 A181 G182 A184 F185 H186 K187 R189 L190 R192 N193 W195 P196 T198 R199 A202 M203 N204 V206 D207

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0042273 ribosomal large subunit biogenesis
• GO:1990145 maintenance of translational fidelity

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:0022626 cytosolic ribosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7of1, PDBe:7of1, PDBj:7of1
• PDBsum: 7of1
• PubMed: 34813592
• UniProt: P0CX45|RL2A_YEAST Large ribosomal subunit protein uL2A (Gene Name=RPL2A)