Structure of PDB 7nis Chain A Binding Site BS01

Receptor Information

>7nis Chain A (length=513) Species: 88776 (Influenza A virus (A/Brevig Mission/1/1918(H1N1)))

RFEITGTMRRLADQSLPPNFSSLENFRAYVDGFEPNGYIEGKLSQMSKEV
NARIEPFLKTTPRPLRLPDGPPCSQRSKFLLMDALKLSIEDPSHEGEGIP
LYDAIKCMRTFFGWKEPNVVKPHEKGINPNYLLAWKQVLAELQDIENEEK
IPKTKNMKKTSQLKWALGENMAPEKVDFDDCKDVSDLKQYDSDEPELRSL
ASWIQSEFNKACELTDSSWIELDEIGEDVAPIEHIASMRRNYFTAEVSHC
RATEYIMKGVYINTALLNASCAAMDDFQLIPMISKCRTKEGRRKTNLYGF
IIKGRSHLRNDTDVVNFVSMEFSLTDPRLEPHKWEKYCVLEIGDMLLRSA
IGQVSRPMFLYVRTNGTSKIKMKWGMEMRRCLLQSLQQIESMIEAESSVK
EKDMTKEFFENKSETWPIGESPKGVEEGSIGKVCRTLLAKSVFNSLYASP
QLEGFSAESRKLLLIVQALRDNLEPGTFDLGGLYEAIEECLINDPWVLLN
ASWFNSFLTHALR

Ligand information

>7nis Chain D (length=15)

aguagaaacaaggcc

.<<....>>......

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7nis Mapping inhibitory sites on the RNA polymerase of the 1918 pandemic influenza virus using nanobodies.
• Resolution: 5.96 Å
• Binding residue (original residue number in PDB): R279 H326 K328 G371 M374 A375 P376 Y393 D394 E397 H510 D516 V517 N519 K539 R566 T567 N568 K574 P653
• Binding residue (residue number reindexed from 1): R76 H123 K125 G168 M171 A172 P173 Y190 D191 E194 H307 D313 V314 N316 K336 R363 T364 N365 K371 P450

Enzymatic activity

• Enzyme Commision number: 3.1.-.-

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0004519 endonuclease activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006351 DNA-templated transcription
• GO:0039523 symbiont-mediated suppression of host mRNA transcription via inhibition of RNA polymerase II activity
• GO:0039694 viral RNA genome replication
• GO:0075523 viral translational frameshifting
• GO:0075526 cap snatching

Cellular Component

• GO:0030430 host cell cytoplasm
• GO:0042025 host cell nucleus

External links

• PDB: RCSB:7nis, PDBe:7nis, PDBj:7nis
• PDBsum: 7nis
• PubMed: 35017564
• UniProt: Q3HM39|PA_I18A0 Polymerase acidic protein (Gene Name=PA)