Structure of PDB 7mjx Chain A Binding Site BS01

Receptor Information

>7mjx Chain A (length=442) Species: 820 (Bacteroides uniformis)

DNKKLFIETYGCQMNVADSEVIASVMQMAGYSVADTLEEADAVFMNTCSI
RDNAEQKILNRLEFFHSLKKKKRGLIVGVLGCMAERVKDDLITNHHVDLV
VGPDAYLTLPELIASVEAGEKAMNVELSTTETYRDVIPSRICGNHISGFV
SIMRGCNNFCTYCIVPYTRGRERSRDVESILNEVADLVAKGYKEVTLLGQ
NVNSYRFEKPDGETITFPMLLRTVAEAAPGVRIRFTTSHPKDMSDETLQV
IADMPNVCKHIHLPVQSGSSRILKLMNRKYDREWYMDRVAAIRRIIPDCG
LSTDIFSGFHSETEEDHQLSLSLMEECGYDSAFMFKYSERPGTHASKHLP
DDVPEEVKIRRLNEIIALQNRLSAEANARCVGKTYEVLVEGVSKRSRDQL
FGRTEQNRVVVFDRGTHRVGDFVMVKVTESSSATLKGEEVAG

Ligand information

>7mjx Chain M (length=13)

ggacugaaaaucc

<<<.......>>>

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7mjx Structural basis for tRNA methylthiolation by the radical SAM enzyme MiaB.
• Resolution: 1.5 Å
• Binding residue (original residue number in PDB): G26 C27 Q28 C63 R66 N68 K72 T252 D319 F348 M349 F350 I381 K409 R410 R418 V424 S446 S447 A448 T449 K451
• Binding residue (residue number reindexed from 1): G11 C12 Q13 C48 R51 N53 K57 T237 D304 F333 M334 F335 I366 K394 R395 R403 V409 S431 S432 A433 T434 K436

Enzymatic activity

• Enzyme Commision number: 2.8.4.3: tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase.

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0016740 transferase activity
• GO:0035596 methylthiotransferase activity
• GO:0046872 metal ion binding
• GO:0051536 iron-sulfur cluster binding
• GO:0051538 3 iron, 4 sulfur cluster binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:0006400 tRNA modification
• GO:0008033 tRNA processing
• GO:0035600 tRNA methylthiolation

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:7mjx, PDBe:7mjx, PDBj:7mjx
• PDBsum: 7mjx
• PubMed: 34526715
• UniProt: A0A174NUT3