Structure of PDB 7mi5 Chain A Binding Site BS01

Receptor Information

>7mi5 Chain A (length=553) Species: 243231 (Geobacter sulfurreducens PCA)

GSIPLIPVRMLNEHVYCPRLAYLMWVQGEFSHNEFTVDGVIRHRRVDAGG
GVLPSETQEDSRIHARSVSLSSERLGITAKIDLVEGEGAYVSPVDYKRGK
RPHVAGGAYEPERVQLCAQGLLLREHGFASDGGALYFVASRERVPVAFDD
ELIGRTLAAIDEMGRTALSGTMPPPLEDSPKCPRCSLVGICLPDEVRFLS
HLSVEPRPIIPADGRGLPLYVQSPKAYVRKDGDCLVIEEERVRVAEARLG
ETSQVALFGNATLTTAALHECLRREIPVTWLSYGGWFMGHTVSTGHRNVE
TRTYQYQRSFDPETCLNLARRWIVAKIANCRTLLRRNWRGEGDEAKAPPG
LLMSLQDDMRHAMRAPSLEVLLGIEGASAGRYFQHFSRMLRGGDGEGMGF
DFTTRNRRPPKDPVNALLSFAYAMLTREWTVALAAVGLDPYRGFYHQPRF
GRPALALDMMEPFRPLIADSTVLMAINNGEIRTGDFVRSAGGCNLTDSAR
KRFIAGFERRMEQEVTHPIFKYTISYRRLLEVQARLLTRYLSGEIPAYPN
FVT

Ligand information

>7mi5 Chain G (length=26)

accatcgtgaggcctcagctacgact

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7mi5 Mechanism for Cas4-assisted directional spacer acquisition in CRISPR-Cas.
• Resolution: 3.57 Å
• Binding residue (original residue number in PDB): R234 K235 D236 G237 T269
• Binding residue (residue number reindexed from 1): R229 K230 D231 G232 T264

Enzymatic activity

• Enzyme Commision number: 3.1.-.-
  3.1.12.1: 5' to 3' exodeoxyribonuclease (nucleoside 3'-phosphate-forming).

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0004519 endonuclease activity
• GO:0004527 exonuclease activity
• GO:0046872 metal ion binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:0043571 maintenance of CRISPR repeat elements
• GO:0051607 defense response to virus

External links

• PDB: RCSB:7mi5, PDBe:7mi5, PDBj:7mi5
• PDBsum: 7mi5
• PubMed: 34588691
• UniProt: Q74H36|CS4F1_GEOSL CRISPR-associated exonuclease Cas4/endonuclease Cas1 fusion (Gene Name=cas4-cas1)