Structure of PDB 7mhz Chain A Binding Site BS01

Receptor Information

>7mhz Chain A (length=491) Species: 9606 (Homo sapiens)

MLPRWELALYLLASLGFHFYSFYEVYKVSREHEEELDQEFELETDTLFGG
LKKDATDFEWSFWMEWGKQWLVWLLLGHMVVSQMATLLARKHRPWILMLY
GMWACWCVLGTPGVAMVLLHTTISFCVAQFRSQLLTWLCSLLLLSTLRLQ
GVEEVKRRWYKTENEYYLLQFTLTVRCLYYTSFSLELCWQQLPAASTSYS
FPWMLAYVFYYPVLHNGPILSFSEFIKQMQQQEHDSLKASLCVLALGLGR
LLCWWWLAELMAHLMYMHAIYSSIPLLETVSCWTLGGLALAQVLFFYVKY
LVLFGVPALLMRLDGLTPPALPRCVSTMFSFTGMWRYFDVGLHNFLIRYV
YIPVGGSQHGLLGTLFSTAMTFAFVSYWHGGYDYLWCWAALNWLGVTVEN
GVRRLVETPCIQDSLARYFSPQARRRFHAALASCSTSMLILSNLVFLGGN
EVGKTYWNRIFIQGWPWVTLSVLGFLYCYSHVGIAWAQTYA

Ligand information

>7mhz Chain B (length=8) Species: 9606 (Homo sapiens)

CGPGRGFG

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7mhz Substrate and product complexes reveal mechanisms of Hedgehog acylation by HHAT.
• Resolution: 3.2 Å
• Binding residue (original residue number in PDB): F58 E59 Y167 F171 T174 N216 Y300 D339 H379 Y382 Y384 F446
• Binding residue (residue number reindexed from 1): F58 E59 Y167 F171 T174 N216 Y300 D339 H379 Y382 Y384 F446

Enzymatic activity

• Enzyme Commision number: 2.3.1.-

Gene Ontology

Molecular Function

• GO:0005515 protein binding
• GO:0005525 GTP binding
• GO:0008374 O-acyltransferase activity
• GO:0016409 palmitoyltransferase activity
• GO:0016746 acyltransferase activity

Biological Process

• GO:0007224 smoothened signaling pathway
• GO:0018009 N-terminal peptidyl-L-cysteine N-palmitoylation

Cellular Component

• GO:0000139 Golgi membrane
• GO:0005783 endoplasmic reticulum
• GO:0005789 endoplasmic reticulum membrane
• GO:0005794 Golgi apparatus
• GO:0016020 membrane

External links

• PDB: RCSB:7mhz, PDBe:7mhz, PDBj:7mhz
• PDBsum: 7mhz
• PubMed: 34112694
• UniProt: Q5VTY9|HHAT_HUMAN Protein-cysteine N-palmitoyltransferase HHAT (Gene Name=HHAT)