Structure of PDB 7lt5 Chain A Binding Site BS01

Receptor Information

>7lt5 Chain A (length=568) Species: 272563 (Clostridioides difficile 630)

SQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKTLKNHDIIKNP
YPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWTVDNIHRH
ILNYCIYGADIDEKAISILKDSLTNKKVESDIKINLFCCDSLKKKWRYKF
DYIVGNPPYIGHKKLEKKYKKFLLEKYSEVYKDKADLYFCFYKKIIDILK
QGGIGSVITPRYFLESLSGKDLREYIKSNVNVQEIVDFLGANIFKNIGVS
SCILTFDKKKTKETYIDVFKIKNEDICINKFETLEELLKSSKFEHFNINQ
RLLSDEWILVNKDDETFYNKIQEKCKYSLEDIAISFQGIITGCDKAFILS
KDDVKLNLVDDKFLKCWIKSKNINKYIVDKSEYRLIYSNDIDNENTNKRI
LDEIIGLYKTKLENRRECKSGIRKWYELQWGREKLFFERKKIMYPYKSNE
NRFAIDYDNNFSSADVYSFFIKEEYLDKFSYEYLVGILNSSVYDKYFKIT
AKKMSKNIYDYYPNKVMKIRIFRDNNYEEIENLSKQIISILLNKSIDKGK
VEKLQIKMDNLIMDSLGI

Ligand information

>7lt5 Chain E (length=13)

tgggactttttga

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7lt5 Clostridioides difficile specific DNA adenine methyltransferase CamA squeezes and flips adenine out of DNA helix.
• Resolution: 2.54 Å
• Binding residue (original residue number in PDB): H171 K172 K179 D192 K193 I349 D353 K378 K380 R425 Q438 W439 Y455 K456 I517
• Binding residue (residue number reindexed from 1): H162 K163 K170 D183 K184 I340 D344 K369 K371 R416 Q429 W430 Y446 K447 I508

Enzymatic activity

• Enzyme Commision number: 2.1.1.72: site-specific DNA-methyltransferase (adenine-specific).

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0008168 methyltransferase activity
• GO:0009007 site-specific DNA-methyltransferase (adenine-specific) activity

Biological Process

• GO:0006304 DNA modification
• GO:0032259 methylation

External links

• PDB: RCSB:7lt5, PDBe:7lt5, PDBj:7lt5
• PDBsum: 7lt5
• PubMed: 34103525
• UniProt: Q183J3