Structure of PDB 7lni Chain A Binding Site BS01

Receptor Information

>7lni Chain A (length=547) Species: 272563 (Clostridioides difficile 630)

ASGIYYTPKIIVDYIVKKTLKNHDIIKNPYPRILDISCGCGNFLLEVYDI
LYDLFEENIYELKKKYDENYWTVDNIHRHILNYCIYGADIDEKAISILKD
SLTNKKDESDIKINLFCCDSLKKKWRYKFDYIVGNPPYIGHKKLEKKYKK
FLLEKYSEVYKDKADLYFCFYKKIIDILKQGGIGSVITPRYFLESLSGKD
LREYIKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYIDVFKI
KNEDICINKFETLEELLKSSKFEHFNINQRLLSDEWILVNKDDETFYNKI
QEKCKYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSK
NINKYIVDKSEYRLIYSNDIDNENTNKRILDEIIGLYKTKLENRRECKSG
IRKWYELQWGREKLFFERKKIMYPYKSNENRFAIDYDNNFSSADVYSFFI
KEEYLDKFSYEYLVGILNSSVYDKYFKITAKKMSKNIYDYYPNKVMKIRI
FRDNNYEEIENLSKQIISILLNKSIDKGKVEKLQIKMDNLIMDSLGI

Ligand information

>7lni Chain E (length=14)

atgggactttttga

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7lni Clostridioides difficile specific DNA adenine methyltransferase CamA squeezes and flips adenine out of DNA helix.
• Resolution: 2.68 Å
• Binding residue (original residue number in PDB): H171 K172 K176 K179 D192 K193 I349 G351 D353 K378 K380 R425 Q438 W439 Y455 K456 I517
• Binding residue (residue number reindexed from 1): H141 K142 K146 K149 D162 K163 I319 G321 D323 K348 K350 R395 Q408 W409 Y425 K426 I487

Enzymatic activity

• Enzyme Commision number: 2.1.1.72: site-specific DNA-methyltransferase (adenine-specific).

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0008168 methyltransferase activity
• GO:0009007 site-specific DNA-methyltransferase (adenine-specific) activity

Biological Process

• GO:0006304 DNA modification
• GO:0032259 methylation

External links

• PDB: RCSB:7lni, PDBe:7lni, PDBj:7lni
• PDBsum: 7lni
• PubMed: 34103525
• UniProt: Q183J3