Structure of PDB 7l6r Chain A Binding Site BS01

Receptor Information

>7l6r Chain A (length=300) Species: 2697049 (Severe acute respiratory syndrome coronavirus 2)

SNSSQAWQPGVAMPNLYKMQRMLLEKCDLQNYGDSATLPKGIMMNVAKYT
QLCQYLNTLTLAVPYNMRVIHFGAGSDKGVAPGTAVLRQWLPTGTLLVDS
DLNDFVSDADSTLIGDCATVHTANKWDLIISDMYDPKTKNVTKENDSKEG
FFTYICGFIQQKLALGGSVAIKITEHSWNADLYKLMGHFAWWTAFVTNVN
ASSSEAFLIGCNYLGKPREQIDGYVMHANYIFWRNTNPIQLSSYSLFDMS
KFPLKLRGTAVMSLKEGQINDMILSLLSKGRLIIRENNRVVISSDVLVNN

Ligand information

>7l6r Chain C (length=6)

gauuaa

......

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7l6r Mn 2+ coordinates Cap-0-RNA to align substrates for efficient 2'- O -methyl transfer by SARS-CoV-2 nsp16.
• Resolution: 1.98 Å
• Binding residue (original residue number in PDB): C6823 D6824 L6825 Y6828 S6831 A6832 L6834 M6840 N6841 K6844 D6873 K6874 Y6930 K6935 K6968 T6970 E6971 H6972 S6999 S7000
• Binding residue (residue number reindexed from 1): C27 D28 L29 Y32 S35 A36 L38 M44 N45 K48 D77 K78 Y134 K139 K172 T174 E175 H176 S203 S204

Enzymatic activity

• Enzyme Commision number: 2.1.1.56: mRNA (guanine-N(7))-methyltransferase.
  2.1.1.57: methyltransferase cap1.
  2.7.7.48: RNA-directed RNA polymerase.
  2.7.7.50: mRNA guanylyltransferase.
  3.1.13.-
  3.4.19.12: ubiquitinyl hydrolase 1.
  3.4.22.-
  3.4.22.69: SARS coronavirus main proteinase.
  3.6.4.12: DNA helicase.
  3.6.4.13: RNA helicase.
  4.6.1.-

Gene Ontology

Molecular Function

• GO:0004483 mRNA (nucleoside-2'-O-)-methyltransferase activity

External links

• PDB: RCSB:7l6r, PDBe:7l6r, PDBj:7l6r
• PDBsum: 7l6r
• PubMed: 34131072
• UniProt: P0DTD1|R1AB_SARS2 Replicase polyprotein 1ab (Gene Name=rep)