Structure of PDB 7aoe Chain A Binding Site BS01

Receptor Information

>7aoe Chain A (length=1401) Species: 284812 (Schizosaccharomyces pombe 972h-)

MNIAQPVSSEIKSVKFGIYDVDDVEKISVKQIVNPVLLDNLNHPTNGGLY
DLALGPYLKNSVCATCHLDERYCPGHFGHIVLPIPAYHPLFFSQMYNLLR
STCLYCHHFKLSKVKVHLFFCRLKLLDYGLLNESEMVENVSLAKDAATLM
RIRDEFVAKSIADSRQLTTLLLHERKKVVRAFYHAISSRKQCDNCQSFSP
NFRKEGFAKIFEIPLSGKNLQFKYMTSTEVRNHLRRLFVKENVVLSRLYP
ASADMFFLQNIAVPPTRFRPASKMGDEVHENIQNELLTRILQSSIQIASL
SKDSTVEERRSRAFELLINAFVQLQHDVNSLIDSNRNPPPGIKQILEKKE
GLFRKHMMGKRVNYAARSVISPDPNIETNEIGVPPVFATKLTYPEPVTLY
NFNEMRNAVINGPHKWPGASHIQNEDGTLISLMPLTIEQRTALANQLLTP
QSNLISSPYSYSRLINTNKKVYRHVRNGDMLILNRQPTLHKPSMMAHKAR
ILPGEKTIRMHYANCNSYNADFDGDEMNMHFPQSTNARSEAQFIANTDSQ
YLVPTSGDPLRGLIQDHVVMGVWLTCKDTFYTRDEYQQLLFQALKPDETG
MYGRIKTLPPAIQRPGIYWTGKQIISSVLLNLKPSDRPGLNLKSKAKVPG
KYWSPDSEEGSVLFDDGELLCGILDKSSFGASAFGLVHSVHELYGPDIAG
RLLSVLSRLFTAYAQMRGFTCRMDDLRLDEQGDNWRRQLLENGKSFGLEA
ASEYVGLSTDSPIALLNANLEEVYRDDEKLQGLDAAMKGKMNGLTSSIIN
KCIPDGLLTKFPYNHMQTMTVSGAKGSNVNVSQISCLLGQQELEGRRVPL
MVSGKSLPSFVPYETSAKSGGFIASRFLTGIAPQEYYFHCMAGREGLIDT
AVKTSRSGYLQRCLMKHLEGLCVQYDHTVRDSDGSIVQFHYGEDSLDVTK
QKHLTQFEFSAKNYKSLIQKYKVKSVLSAVDSETASSYAKKALKKPYKYD
PVLDKYPPSRYLGSVSEKFQRAVDEYTQKNPDKLIAESKLDDSLLNESKF
KALMQLRYQQSLVDPGESVGVLASQSIGEPSTQMTLNTNVTLGIPRLREI
IMTASANIQTPTMTLRLNDGVSDKRASAFCKEVNKLVLSEVVRQVRVTEK
TYAIRLDLYSRDEYQDEYGVLQEEIESTFSNRFLKILNRIIKSYLAKSKQ
NCKHLDFDYVNGEWATVELVFPINTEKLLMVSLVEKACSETVIHEIPGIT
RCFSKPDTVPKVITEGVNLKAIWEFYNEISMNDIYTNDIAAILRIYGVEA
ARNAIVHEVSSVFGVYGIAVDPRHLSLIADYMTFEGGYKAFNRMGIEYNT
SPFAKMSFETTCHFLTEAALRGDVDDLSNPSSRLVVGRVGNFGTGSFDIF
T

Ligand information

>7aoe Chain T (length=25)

aagctcaagtacttaagcctggtca

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7aoe Conserved strategies of RNA polymerase I hibernation and activation.
• Resolution: 3.9 Å
• Binding residue (original residue number in PDB): K471 R489 Q608 T1026 S1027 G1030 R1034
• Binding residue (residue number reindexed from 1): K349 R367 Q486 T904 S905 G908 R912

Enzymatic activity

• Enzyme Commision number: 2.7.7.6: DNA-directed RNA polymerase.

Gene Ontology

Molecular Function

• GO:0001054 RNA polymerase I activity
• GO:0003677 DNA binding
• GO:0003899 DNA-directed 5'-3' RNA polymerase activity
• GO:0005515 protein binding
• GO:0016779 nucleotidyltransferase activity
• GO:0034062 5'-3' RNA polymerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006351 DNA-templated transcription
• GO:0006362 transcription elongation by RNA polymerase I

Cellular Component

• GO:0000428 DNA-directed RNA polymerase complex
• GO:0005634 nucleus
• GO:0005730 nucleolus
• GO:0005736 RNA polymerase I complex
• GO:0005829 cytosol

External links

• PDB: RCSB:7aoe, PDBe:7aoe, PDBj:7aoe
• PDBsum: 7aoe
• PubMed: 33536435
• UniProt: P15398|RPA1_SCHPO DNA-directed RNA polymerase I subunit rpa1 (Gene Name=rpa1)