Structure of PDB 6z8k Chain A Binding Site BS01

Receptor Information

>6z8k Chain A (length=2158)

MDYQEYQQFLARINTARDACVAKDIDVDLLMARHDYFGRELCKSLNIEYR
NDVPFIDIILDIRPEVDPLTIDAPHITPDNYLYINNVLYIIDYKVSVSNE
SSVITYDKYYELTRDISDRLSIPIEIVIIRIDPVSRDLHINSDRFKELYP
TIVVDINFNQFFDLKQLLYEKFGDDEEFLLKVAHGDFTLTAPWCKTGCPE
FWKHPIYKEFKMSMPVPERRLFEESVKFNAYESERWNTNLVKIREYTKKD
YSEHISKSAKNIFLASGFYKQPNKNEISEGWTLMVERVQDQREISKSLHD
QKPSIHFIWGAHNPGNSNNATFKLILLSKSLQSIKGISTYTEAFKSLGKM
MDIGDKAIEYEEFCMSLKSKARSSWKQIMNKKLEPKQINNALVLWEQQFM
INNDLIDKSEKLKLFKNFCGIGKSKPKILDFDDANMYLASLTMMEQSKKI
LSKSNGLKPDNFILNEFGSRIKDANKETYDNMHKIFETGYWQCISDFSTL
MKNILSVSQYNRHNTFRIAMCANNNVFAIVFPATVVYSIIVLHKEEENIF
NPGCLHGTFKCMNGYISISRAIRLDKERCQRIVSSPGLFLTTCLLFKHDN
PTLVMSDIMNFSIYTSLSITKSVLSLTEPARYMIMNSLAISSNVKDYIAE
KFSPYTKTLFSVYMTRLIKNACFDAYDQRQRVQLRDIYLSDYDITQKGIK
DNRELTSIWFPGSVTLKEYLTQIYLPFYFNAKGLHEKHHVMVDLAKTILE
IECEQRENIKEIWSTNCTKQTVNLKILIHSLCKNLLADTSRHNHLRNRIE
NRNNFRRSITTISTFTSSKSCLKIGDFRKEKELQSVKQKKILEVQSRKNY
EMLRNAMPNYTDYISTKVFDRLYELLDKKVLTDKPVIEQIMDMMIDHKKF
YFTFFNKGQKTSKDREIFVGEYEAKMCMYAVERIAKERCKLNPDEMISEP
GDGKLKVLEQKSEQEIRFLVETTRQKNREIDEAIELGKIEKLSLGKAKGL
KMEINADMSKWSAQDVFYKYFWLIALDPILYPQEKERILYFMCNYMDKEL
ILPDELLFNLLDQKVAYQNDIIATMTNQLNSNTVLIKRNWLQGNFNYTSS
YVHSCAMSVYKEILKEAITLLDGSILVNSLVHSDDNQTSITIVQDKMEND
KIIDFAMKEFERACLTFGCQANMKKTYVTNCIKEFVSLFNLYGEPFSIYG
RFLLTSVGDCAYIGPYEDLASRISSAQTAIKHGCPPSLAWVSIAISHWMT
SLTYNMLPGQSNDPIDYFPAENRKDIPIELNGVLDAPLSMISTVGLESGN
LYFLIKLLSKYTPVMQKRESVVNQIAEVKNWKVEDLTDNEIFRLKILRYL
VLDAEMDPSDIMGETSDMRGRSILTPRKFTTAGSLRKLYSFSKYQDRLSS
PGGMVELFTYLLEKPELLVTKGEDMKDYMESVIFRYNSKRFKESLSIQNP
AQLFIEQILFSHKPVIDFSGIRDKYINLGKVTFTEAYRLLMRDLSSLELT
NDDIQVIYSYIILNDPMMITIANTHILSIYGSPQRRMGMSCSTMPEFRNL
KLIHHSPALVLRAYSKNNPDPTEMARDLVHLKEFVENTNLEEKMKVRIAM
NEAEKGQRDIVFELKEMTRFYQVCYEYVKSHKIKVFILPAKSYTTTDFCS
LMQGNLIKDKEWYTVHYLKQILSGGHKAIMQHNATSEQNIAFECFKLITH
FADSFIDSLSRSAFLQLIIDEFSYKDVKVSKLYDIIKNGYNRTDFIPLLF
RTGDLRQADLDKYDAMKSHERVTWNDWINLTITGYNRSITIIGEDNKLTY
AELCLTRKTPENITISGRKLLGSRHGLKFENMSKIQNYYITYRKKDRHQF
VYQIHSHESITRRNEEHMAIRTRIYNEITPVCVVNVAEVDGDQRILIRSL
DYLNNDIFSLSRIKVGLDEFATIKKAHFSKMVSFEGPPIKTGLLDLTELM
KSQDLLNLNYDNIRNSNLISFSKLICCEGSDNINDGLEFLSDDPMNFTEG
EAIHSTPIFNIYYSKRGERHMTYRNAIKLLIERETKIFEEAFTFSENGFI
SPENLGCLEAVVSLIKLLKTNEWSTVIDKCIHICLIKNGMDHMYHSFDVP
KCFMGNPITRDINWVMFREFINSLPGTDIPPWNVMTENFKKKCIALINSK
FSEFTKLM

Ligand information

>6z8k Chain C (length=10)

aguagugugc

.<<.....>>

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6z8k Pre-initiation and elongation structures of full-length La Crosse virus polymerase reveal functionally important conformational changes.
• Resolution: 3.02 Å
• Binding residue (original residue number in PDB): Q291 R292 K302 C419 G420 K439 P440 R592 A593 R595 R600 T642 K643 Y677 E758 H760 H761 K768 L1113 Q1116 V1118 Y1120 D1123
• Binding residue (residue number reindexed from 1): Q291 R292 K302 C419 G420 K425 P426 R570 A571 R573 R578 T620 K621 Y655 E736 H738 H739 K746 L1060 Q1063 V1065 Y1067 D1070

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.
  3.1.-.-

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003723 RNA binding
• GO:0003968 RNA-dependent RNA polymerase activity
• GO:0016787 hydrolase activity
• GO:0034062 5'-3' RNA polymerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0001172 RNA-templated transcription
• GO:0006351 DNA-templated transcription
• GO:0019079 viral genome replication
• GO:0039694 viral RNA genome replication

Cellular Component

• GO:0044165 host cell endoplasmic reticulum
• GO:0044172 host cell endoplasmic reticulum-Golgi intermediate compartment
• GO:0044177 host cell Golgi apparatus
• GO:0044423 virion component

External links

• PDB: RCSB:6z8k, PDBe:6z8k, PDBj:6z8k
• PDBsum: 6z8k
• PubMed: 32681014
• UniProt: A5HC98|L_BUNLC RNA-directed RNA polymerase L (Gene Name=L)