Structure of PDB 6x50 Chain A Binding Site BS01

Receptor Information
>6x50 Chain A (length=1143) Species: 562 (Escherichia coli) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
YRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVLIAPDMQNALR
LHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQRG
VLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVD
QVMEHGEYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLE
EVEAINLLPAHEFPTDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLP
AGIEYWQPLFFSEPLPPLFSYFPANTLLVNTGDLETSAERFQADTLARFE
NRGVDPMRPLLPPQSLWLRVDELFSELKNWPRVQLKTEHLPTKAANANLG
FQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESEGRREALGELLAR
IKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDTVRNLALICESDLLGERV
ARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGI
TGEYLMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRAR
QKAAEKVRDVAAELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPD
QAQAINAVLSDMCQPLAMDRLVCGDVGFGKTEVAMRAAFLAVDNHKQVAV
LVPTTLLAQQHYDNFRDRFANWPVRIEMISRFRSAKEQTQILAEVAEGKI
DILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKERIKAMRANVDILTL
TATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSMVVREAILR
EILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVM
NDFHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVG
RSHHQAYAWLLTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRG
AGELLGEEQSGSMETIGFSLYMELLENAVDALKAGREPSLEDLTSQQTEV
ELRMPSLLPDDFIPDVNTRLSFYKRIASAKTENELEEIKVELIDRFGLLP
DPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIEFAEKNHVNPAWLIGLL
QKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENAI
Ligand information
>6x50 Chain P (length=55) [Search DNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
gggtattcgccgcgtacctctcctagcccgcaagtatcctattccttgca
gcggt
Receptor-Ligand Complex Structure
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PDB6x50 Structural basis for transcription complex disruption by the Mfd translocase.
Resolution3.3 Å
Binding residue
(original residue number in PDB)		K368 D460 S461 A553 R554 K556 T658 T659 R685 T710 H711 K712 N817 V819 G843 Q844 T868 I870
Binding residue
(residue number reindexed from 1)		K364 D456 S457 A549 R550 K552 T654 T655 R681 T706 H707 K708 N813 V815 G839 Q840 T864 I866
Enzymatic activity
Enzyme Commision number 3.6.4.-
Gene Ontology
Molecular Function
GO:0003676 nucleic acid binding
GO:0003677 DNA binding
GO:0003684 damaged DNA binding
GO:0004386 helicase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0015616 DNA translocase activity
GO:0016787 hydrolase activity
GO:0043175 RNA polymerase core enzyme binding
Biological Process
GO:0000716 transcription-coupled nucleotide-excision repair, DNA damage recognition
GO:0006281 DNA repair
GO:0006283 transcription-coupled nucleotide-excision repair
GO:0006294 nucleotide-excision repair, preincision complex assembly
GO:0006355 regulation of DNA-templated transcription
GO:0006974 DNA damage response
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:1990391 DNA repair complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:6x50, PDBe:6x50, PDBj:6x50
PDBsum6x50
PubMed33480355
UniProtP30958|MFD_ECOLI Transcription-repair-coupling factor (Gene Name=mfd)

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