Structure of PDB 6uit Chain A Binding Site BS01

Receptor Information

>6uit Chain A (length=545) Species: 11706 (HIV-1 M:B_HXB2R)

PISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKI
GPENPYNTPVFAIKKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPHPAGL
KKKKSVTVLDVGDAYFSVPLDEDFRKYTAFTIPGIRYQYNVLPQGWKGSP
AIFQSSMTKILEPFRKQNPDIVIYQYMDDLYVGSDLEIGQHRTKIEELRQ
HLLRWGLTTPDKKHQKEPPFLWMGYELHPDKWTVQPIVLPEKDSWTVNDI
CKLVGKLNWASQIYPGIKVRQLSKLLRALTEVIPLTEEAELELAENREIL
KEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMRGA
HTNDVKQLTEAVQKITTESIVIWGKTPKFKLPIQKETWETWWTEYWQATW
IPEWEFVNTPPLVKLWYQLEKEPIVGAETFYVDGAANRETKLGKAGYVTN
RGRQKVVTLTDTTNQKTELQAIYLALQDSGLEVNIVTDSQYALGIIQAQP
DQSESELVNQIIEQLIKKEKVYLAWVPAHKGIGGNEQVDKLVSAG

Ligand information

>6uit Chain P (length=18)

gtccctgttcgggcgccc

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6uit Elucidating molecular interactions ofL-nucleotides with HIV-1 reverse transcriptase and mechanism of M184V-caused drug resistance.
• Resolution: 2.80607 Å
• Binding residue (original residue number in PDB): Y183 M184 D185 M230 G231 C258 K259 G262 K263 W266 R358 G359 H361 R448 K451 T473 Q475 Y501
• Binding residue (residue number reindexed from 1): Y176 M177 D178 M223 G224 C251 K252 G255 K256 W259 R348 G349 H351 R438 K441 T463 Q465 Y491

Enzymatic activity

• Enzyme Commision number: 2.7.7.-
  2.7.7.49: RNA-directed DNA polymerase.
  2.7.7.7: DNA-directed DNA polymerase.
  3.1.-.-
  3.1.13.2: exoribonuclease H.
  3.1.26.13: retroviral ribonuclease H.
  3.4.23.16: HIV-1 retropepsin.

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003964 RNA-directed DNA polymerase activity
• GO:0004523 RNA-DNA hybrid ribonuclease activity

Biological Process

• GO:0006278 RNA-templated DNA biosynthetic process

External links

• PDB: RCSB:6uit, PDBe:6uit, PDBj:6uit
• PDBsum: 6uit
• PubMed: 31872074
• UniProt: P04585|POL_HV1H2 Gag-Pol polyprotein (Gene Name=gag-pol)