Structure of PDB 6uis Chain A Binding Site BS01

Receptor Information

>6uis Chain A (length=547) Species: 11706 (HIV-1 M:B_HXB2R)

PISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKI
GPENPYNTPVFAIKKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPHPAGL
KKKKSVTVLDVGDAYFSVPLDEDFRKYTAFTIPSIRYQYNVLPQGWKGSP
AIFQSSMTKILEPFRKQNPDIVIYQYVDDLYVGSDLEIGQHRTKIEELRQ
HLLRWGLTTPDKKHQKEPPFLWMGYELHPDKWTVQPIVLPEKDSWTVNDI
CKLVGKLNWASQIYPGIKVRQLSKLLRGTKALTEVIPLTEEAELELAENR
EILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARM
RGAHTNDVKQLTEAVQKITTESIVIWGKTPKFKLPIQKETWETWWTEYWQ
ATWIPEWEFVNTPPLVKLWYQLEKEPIVGAETFYVDGAANRETKLGKAGY
VTNRGRQKVVTLTDTTNQKTELQAIYLALQDSGLEVNIVTDSQYALGIIQ
AQPDQSESELVNQIIEQLIKKEKVYLAWVPAHKGIGGNEQVDKLVSA

Ligand information

>6uis Chain P (length=18)

gtccctgttcgggcgccc

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6uis Elucidating molecular interactions ofL-nucleotides with HIV-1 reverse transcriptase and mechanism of M184V-caused drug resistance.
• Resolution: 2.74834 Å
• Binding residue (original residue number in PDB): Y183 M230 G231 C258 K259 G262 K263 W266 L289 R358 H361 R448 K451 T473 Q475 Y501
• Binding residue (residue number reindexed from 1): Y176 M223 G224 C251 K252 G255 K256 W259 L282 R351 H354 R441 K444 T466 Q468 Y494

Enzymatic activity

• Enzyme Commision number: 2.7.7.-
  2.7.7.49: RNA-directed DNA polymerase.
  2.7.7.7: DNA-directed DNA polymerase.
  3.1.-.-
  3.1.13.2: exoribonuclease H.
  3.1.26.13: retroviral ribonuclease H.
  3.4.23.16: HIV-1 retropepsin.

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003964 RNA-directed DNA polymerase activity
• GO:0004523 RNA-DNA hybrid ribonuclease activity

Biological Process

• GO:0006278 RNA-templated DNA biosynthetic process

External links

• PDB: RCSB:6uis, PDBe:6uis, PDBj:6uis
• PDBsum: 6uis
• PubMed: 31872074
• UniProt: P04585|POL_HV1H2 Gag-Pol polyprotein (Gene Name=gag-pol)