Structure of PDB 6o0y Chain A Binding Site BS01

Receptor Information
>6o0y Chain A (length=1146) Species: 1314 (Streptococcus pyogenes) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
KKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALL
FDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLR
LIYLALAHMIKFRGHFLIEGDLEITKAPLSASMIKRYDEHHQDLTLLKAL
VRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEE
LLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNRE
KIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASA
QSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPA
FLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFN
ASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKT
YAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGF
ANRNFMQLIHDDSLTFKEDIQKAQDSLHEHIANLAGSPAIKKGILQTVKV
VDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGS
QILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVP
QSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLIT
QRKFDNLTKAERGGLSEAGFIKRQLVETRQITKHVAQILDSRMNTKYDEN
DKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTA
LIKKYKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLI
ARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIME
RSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGEL
QKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEII
EQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGA
PAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLG
Ligand information
>6o0y Chain B (length=98) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
ggcgcauaaagaugagacgcguuuuagagcuagaaauagcaaguuaaaau
aaggcuaguccguuaucaacuugaaaaaguggcaccgagucggugcuu
....................<<<<<<..<<<<....>>>>....>>>>>>
..<<.....>>.......<<<<....>>>>.<<<<<<...>>>>>>..
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB6o0y Cryo-EM structures reveal coordinated domain motions that govern DNA cleavage by Cas9.
Resolution3.37 Å
Binding residue
(original residue number in PDB)		K3 K30 L35 K44 N46 E57 A59 R63 K65 R66 R69 R70 R74 R75 N77 L101 S104 F105 H116 H129 T134 H160 F164 R165 G166 Y325 H328 H329 K336 R340 F351 F352 I363 Q402 R403 T404 F405 F446 R447 I448 Y450 L455 R457 N459 S460 F462 I473 N588 R661 P731 K735 K742 R753 S777 E779 Y812 D850 S851 I852 P1090 Q1091 G1103 F1105 I1110 P1112 R1122 K1124 M1169 E1225 A1227 Q1272 H1349 Q1350 S1351 Y1356
Binding residue
(residue number reindexed from 1)		K1 K28 L33 K42 N44 E55 A57 R61 K63 R64 R67 R68 R72 R73 N75 L99 S102 F103 H114 H127 T132 H158 F162 R163 G164 Y187 H190 H191 K198 R202 F213 F214 I225 Q264 R265 T266 F267 F308 R309 I310 Y312 L317 R319 N321 S322 F324 I335 N450 R523 P588 K592 K599 R610 S634 E636 Y669 D707 S708 I709 P870 Q871 G883 F885 I890 P892 R902 K904 M949 E1005 A1007 Q1052 H1129 Q1130 S1131 Y1136
Enzymatic activity
Enzyme Commision number 3.1.-.-
Gene Ontology
Molecular Function
GO:0003676 nucleic acid binding
GO:0003677 DNA binding
GO:0003723 RNA binding
GO:0004519 endonuclease activity
GO:0004520 DNA endonuclease activity
GO:0004527 exonuclease activity
GO:0008408 3'-5' exonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0043571 maintenance of CRISPR repeat elements
GO:0051607 defense response to virus

View graph for
Molecular Function
View graph for
Biological Process
External links
PDB RCSB:6o0y, PDBe:6o0y, PDBj:6o0y
PDBsum6o0y
PubMed31285607
UniProtQ99ZW2|CAS9_STRP1 CRISPR-associated endonuclease Cas9/Csn1 (Gene Name=cas9)

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