Structure of PDB 6o0x Chain A Binding Site BS01

Receptor Information
>6o0x Chain A (length=1112) Species: 1314 (Streptococcus pyogenes) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
KKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALL
FDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLR
LIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPIN
AAKAILSARLSKSRRLENLIAQLKKNGLFGNLIALSLGLTPNFKSNFDLA
EDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVN
TEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGY
AGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGS
IPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLP
KHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKV
TVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDN
EENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGW
GRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQ
KAQDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEQI
TKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVRE
INNYHHAHDAYLNAVVGTALIKKRDFATVRKVLSMPQVNIVKKTEVQTGG
FSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKS
KKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLF
ELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQ
KQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIRE
QAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITG
LYETRIDLSQLG
Ligand information
>6o0x Chain B (length=98) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
ggcgcauaaagaugagacgcguuuuagagcuagaaauagcaaguuaaaau
aaggcuaguccguuaucaacuugaaaaaguggcaccgagucggugcuu
....................<<<<<<..<<<<....>>>>....>>>>>>
..<<.....>>.......<<<<....>>>>.<<<<<<...>>>>>>..
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB6o0x Cryo-EM structures reveal coordinated domain motions that govern DNA cleavage by Cas9.
Resolution3.28 Å
Binding residue
(original residue number in PDB)		K30 L35 K44 N46 E57 T58 A59 T62 R63 R66 R69 R70 R71 R74 N77 L101 S104 F105 K111 R115 H116 I122 H129 K163 F164 R165 G166 H167 Y325 H328 H329 L332 K336 R340 F352 Y359 Q402 R403 T404 F405 F446 R447 I448 L455 R457 N459 S460 R467 I473 K510 Y515 N588 R661 K735 K742 P1090 G1103 G1104 F1105 I1110 P1112 R1122 K1124 A1227 Q1272 Q1350 S1351 Y1356
Binding residue
(residue number reindexed from 1)		K28 L33 K42 N44 E55 T56 A57 T60 R61 R64 R67 R68 R69 R72 N75 L99 S102 F103 K109 R113 H114 I120 H127 K161 F162 R163 G164 H165 Y316 H319 H320 L323 K327 R331 F343 Y350 Q393 R394 T395 F396 F437 R438 I439 L446 R448 N450 S451 R458 I464 K501 Y506 N579 R652 K721 K728 P836 G849 G850 F851 I856 P858 R868 K870 A973 Q1018 Q1096 S1097 Y1102
Enzymatic activity
Enzyme Commision number 3.1.-.-
Gene Ontology
Molecular Function
GO:0003676 nucleic acid binding
GO:0003677 DNA binding
GO:0003723 RNA binding
GO:0004519 endonuclease activity
GO:0004520 DNA endonuclease activity
GO:0004527 exonuclease activity
GO:0008408 3'-5' exonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0043571 maintenance of CRISPR repeat elements
GO:0051607 defense response to virus

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Molecular Function
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Biological Process
External links
PDB RCSB:6o0x, PDBe:6o0x, PDBj:6o0x
PDBsum6o0x
PubMed31285607
UniProtQ99ZW2|CAS9_STRP1 CRISPR-associated endonuclease Cas9/Csn1 (Gene Name=cas9)

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