Structure of PDB 6m0v Chain A Binding Site BS01

Receptor Information
>6m0v Chain A (length=1030) Species: 322159 (Streptococcus thermophilus LMD-9) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
MGSDLVLGLDIGIGSVGVGILNKVTGEIIHKNSRIFPAAQAENNLVRRTN
RQGRRLARRKKHRRVRLNRLFEESGLITDFTKISINLNPYQLRVKGLTDE
LSNEELFIALKNMVKHRGISYKTPGQIQLERYQTYGQLRGDFTVEKDGKK
HRLINVFPTSAYRSEALRILQTQQEFNPQITDEFINRYLEILTGKRKYYH
GPGNEKSRTDYGRYRTSGETLDNIFGILIGKCTFYPDEFRAAKASYTAQE
FNLLNDLNNLTVPTETKKLSKEQKNQIINYVKNEKAMGPAKLFKYIAKLL
DVADIKGYRIDKSGKAEIHTFEAYRKMKTLETLDIEQMDRETLDKLAYVL
TLNTEREGIQEALEHEFADGSFSQKQVDELVQFRKANSSIFGKGWHNFSV
KLMMELIPELYETSEEQMTILTRLGYIDEKLLTEEIYNPVVAKSVRQAIK
IVNAAIKEYGDFDNIVIEMARETNEDDEKKAIQKIQKANKDEKDAAMLKA
ANQYNGKAELPHSVFHGHKQLATKIRLWHQQGERCLYTGKTISIHDLINN
SNQFEVDAILPLSITFDDSLANKVLVYATANQEKGQRTPYQALDSMDDAW
SFRELKAFVRESKTLSNKKKEYLLTEEDISKFDVIERNLVDTRYASRVVL
NALQEHFRAHKIDTKVSVVRGQFTSQLRRHWGIEKTRDTYHHHAVDALII
AASSQLNLYQHFVDTLKSKEFEDSILFSYQVDSKFNRKISDATIYATRQA
KVGKDKADETYVLGKIKDIYTQDGYDAFMKIYKKDKSKFLMYRHDPQTFE
KVIEPILENYPNKVPCNPFLKYKEEHGYIRKYSKKGNGPEIKSLKYYDSK
LGNHIDITPKDSNNKVVLQSVSPWRADVYFNKTTGKYEILGLKYADLQFE
KGTGTYKISQEKYNDIKKKEGVDSDSEFKFTLYKNDLLLVKDTETKEQQL
FRFLSRTMPKQKHYVELKPYDKQKFEGGEALIKVLGNVANSGQCKKGLGK
SNISIYKVRTDVLGNQHIIKNEGDKPKLDF
Ligand information
>6m0v Chain B (length=71) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
ggugcuaagauuaaucaggauguuuuuguacucgaaagaagcuacaaaga
uaaggcuucaugccgaaauca
.....................<<<<<<<<<.<<....>>...>>>>>>>>
>..<<<.....>>>.......
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB6m0v Catalytic-state structure and engineering of Streptococcus thermophilus Cas9
Resolution3.0 Å
Binding residue
(original residue number in PDB)		Q39 A40 N43 V45 R46 R47 N49 R50 Q51 R53 R54 L55 R57 R58 K60 H61 R63 R65 R68 I84 N85 Y89 K110 K114 H115 R116 G117 Y159 G163 Q164 L165 R166 G167 N182 V183 K222 R223 Y225 G228 P229 G230 N231 K233 S234 T236 Y238 R240 Y241 T260 K270 N279 R338 K341 H425 N426 F427 Q446 Y478 N479 P480 K484 R487 R512 Q527 N530 K531 K534 R567 D609 S610 E681 R682 V685 R692 Q699 R715 Q813 V814 D815 S816 K817 N819 R820 K821 I822 I827 A829 R831 K848 M874 H877 Q880 K922 S924 K925 N928 P930 E931 K933 S934 L935 K936 I948 P950 D952 S953 N954 V957 Q989 F990 K992 Y997
Binding residue
(residue number reindexed from 1)		Q40 A41 N44 V46 R47 R48 N50 R51 Q52 R54 R55 L56 R58 R59 K61 H62 R64 R66 R69 I85 N86 Y90 K111 K115 H116 R117 G118 Y132 G136 Q137 L138 R139 G140 N155 V156 K195 R196 Y198 G201 P202 G203 N204 K206 S207 T209 Y211 R213 Y214 T233 K243 N252 R309 K312 H396 N397 F398 Q417 Y437 N438 P439 K443 R446 R471 Q486 N489 K490 K493 R526 D568 S569 E636 R637 V640 R647 Q654 R670 Q730 V731 D732 S733 K734 N736 R737 K738 I739 I744 A746 R748 K765 M791 H794 Q797 K831 S833 K834 N837 P839 E840 K842 S843 L844 K845 I857 P859 D861 S862 N863 V866 Q898 F899 K901 Y906
Enzymatic activity
Enzyme Commision number 3.1.-.-
Gene Ontology
Molecular Function
GO:0003676 nucleic acid binding
GO:0003677 DNA binding
GO:0003723 RNA binding
GO:0004519 endonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0043571 maintenance of CRISPR repeat elements
GO:0051607 defense response to virus

View graph for
Molecular Function
View graph for
Biological Process
External links
PDB RCSB:6m0v, PDBe:6m0v, PDBj:6m0v
PDBsum6m0v
PubMed
UniProtQ03LF7|CAS9A_STRTD CRISPR-associated endonuclease Cas9 1 (Gene Name=cas9-1)

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