Structure of PDB 6gtd Chain A Binding Site BS01

Receptor Information
>6gtd Chain A (length=1269) Species: 401614 (Francisella tularensis subsp. novicida U112) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
MSIYQEFVNKYSLSKTLRFELIPQGKTLENIKARGLILDDEKRAKDYKKA
KQIIDKYHQFFIEEILSSVCISEDLLQNYSDVYFKLKKSDDDNLQKDFKS
AKDTIKKQISEYIKDSEKFKNLFNQNLIDAKKGQESDLILWLKQSKDNGI
ELFKANSDITDIDEALEIIKSFKGWTTYFKGFHENRKNVYSSNDIPTSII
YRIVDDNLPKFLENKAKYESLKDKAPEAINYEQIKKDLAEELTFDIDYKT
SEVNQRVFSLDEVFEIANFNNYLNQSGITKFNTIIGGKFVNGENTKRKGI
NEYINLYSQQINDKTLKKYKMSVLFKQILSDTDKLEDDSDVVTTMQSFYE
QIAAFKTVEEKSIKETLSLLFDDLKAQKLDLSKIYFKNDKSLTDLSQQVF
DDYSVIGTAVLEYITQQIAPKNQELIAKKTEKAKYLSLETIKLALEEFNK
HRDIDKQCRFEEILANFAAIPMIFDEIAQNKDNLAQISIKYQNQGKKDLL
QASAEDDVKAIKDLLDQTNNLLHKLKIFHISQSEDKANILDKDEHFYLVF
EECYFELANIVPLYNKIRNYITQKPYSDEKFKLNFENSTLANGWDKNKEP
DNTAILFIKDDKYYLGVMNKKNNKIFDDKAIKENKGEGYKKIVYKLLPGA
NKMLPKVFFSAKSIKFYNPSEDILRIRNHSTHTKNGSPQKGYEKFEFNIE
DCRKFIDFYKQSISKHPEWKDFGFRFSDTQRYNSIDEFYREVENQGYKLT
FENISESYIDSVVNQGKLYLFQIYNKDFSAYSKGRPNLHTLYWKALFDER
NLQDVVYKLNGEAELFYRKQSIPKKITHPAKEAIANKNKDNPKKESVFEY
DLIKDKRFTEDKFFFHCPITINFKSSGANKFNDEINLLLKEKANDVHILS
IDRGERHLAYYTLVDGKGNIIKQDTFNIIGNDRMKTNYHDKLAAIEKDRD
SARKDWKKINNIKEMKEGYLSQVVHEIAKLVIEYNAIVVFQDLNKQVYQK
LEKMLIEKLNYLVFKDNEFDKTGGVLRAYQLTAPFETFKKMGKQTGIIYY
VPAGFTSKICPVTGFVNQLYPKYESVSKSQEFFSKFDKICYNLDKGYFEF
SFDYKNFGDKAAKGKWTIASFGSRLINFRNSNWDTREVYPTKELEKLLKD
YSIEYGHGECIKAAICGESDKKFFAKLTSVLNTILQMRNSKTGTELDYLI
SPVADVNGNFFDSRQAPKNMPQDADANGAYHIGLKGLMLLGRIKNNQEGK
KLNLVIKNEEYFEFVQNRN
Ligand information
>6gtd Chain B (length=28) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
aauuucuacuguuguagaugagaaguca
....<..<......>..>..........
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB6gtd Conformational Activation Promotes CRISPR-Cas12a Catalysis and Resetting of the Endonuclease Activity.
Resolution4.24 Å
Binding residue
(original residue number in PDB)		S14 K15 T16 P577 S797 K798 G799 R800 L803 H804 H843 I849 A850 K852 N853 V862 F863 Y865 L867 I868 K869 D870 K871 R872 R948 S986 Q987
Binding residue
(residue number reindexed from 1)		S14 K15 T16 P562 S782 K783 G784 R785 L788 H789 H828 I834 A835 K837 N838 V847 F848 Y850 L852 I853 K854 D855 K856 R857 R933 S971 Q972
Enzymatic activity
Enzyme Commision number 3.1.21.1: deoxyribonuclease I.
4.6.1.22: Bacillus subtilis ribonuclease.
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003723 RNA binding
GO:0004519 endonuclease activity
GO:0004530 deoxyribonuclease I activity
GO:0016829 lyase activity
Biological Process
GO:0006259 DNA metabolic process
GO:0051607 defense response to virus

View graph for
Molecular Function
View graph for
Biological Process
External links
PDB RCSB:6gtd, PDBe:6gtd, PDBj:6gtd
PDBsum6gtd
PubMed30503205
UniProtA0Q7Q2|CS12A_FRATN CRISPR-associated endonuclease Cas12a (Gene Name=cas12a)

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