Structure of PDB 5zwm Chain A Binding Site BS01

Receptor Information
>5zwm Chain A (length=2177) Species: 559292 (Saccharomyces cerevisiae S288C) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
KAEMPPEHLRKIINTHSDMASKMYNTDKKAFLGALKYLPHAILKLLENMP
HPWEQAKEVKVLYHTSGAITFVNETPRVIEPVYTAQWSATWIAMRREKRD
RTHFKRMRFPPFDDDEPPLSYEQHIENIEPLDPINLPLDSQDDEYVKDWL
YDSRPLEEDSKKVNGTSYKKWSFDLPEMSNLYRLSTPLRDEVTDKNYYYL
FDKKSFFNGKALNNAIPGGPKFEPLYPREEEEDYNEFNSIDRVIFRVPIR
SEYKVAFPHLYNSRPRSVRIPWYNNPVSCIIQNDEEYDTPALFFDPSLNP
IGDFTLPEDFAPLLAEEEELILPNTKDAMSLYHSPFPFNRTKGKMVRAQD
VALAKKWFLQHPDEEYPVKVKVSYQKLLKNYVLNELHPTLPTNHNKTKLL
KSLKNTKYFQQTTIDWVEAGLQLCRQGHNMLNLLIHRKGLTYLHLDYNFN
LKPTKTLTTKERKKSRLGNSFHLMRELLKMMKLIVDTHVQFRLGNVDAFQ
LADGIHYILNHIGQLTGIYRYKYKVMHQIRACKDLKHIIYYKFNKNLGKG
PGCGFWQPAWRVWLNFLRGTIPLLERYIGNLITRQFEGRLDAYYDLELRN
SVMDDILEMMPESIRQKKARTILQHLSEAWRCWKANIPWDVPGMPAPIKK
IIERYIKSKADAWVSAAHYNRERIKRGAHVEKTMVKKNLGRLTRLWIKNE
QERQRQIQKNGPEITPEEATTIFSVMVEWLESRSFSPIPFPPLTYKNDTK
ILVLALEDLKDVYASKVRLNASEREELALIEEAYDNPHDTLNRIKKYLLT
QRVFKPVDITMMENYQNISPVYSVDPLEKITDAYLDQYLWYEADQRKLFP
NWIKPSDSEIPPLLVYKWTQGINNLSEIWDVSRGQSAVLLETTLGEMAEK
IDFTLLNRLLRLIVDPNIADYITAKNNVVINFKDMSHVNKYGLIRGLKFA
SFIFQYYGLVIDLLLLGQERATDLAGPANNPNEFMQFKSKEVEKAHPIRL
YTRYLDRIYMLFHFEEDEGEELTDEYLAENPDPNFENSIGYNNRKCWPKD
SRMRLIRQDVNLGRAVFWEIQSRVPTSLTSIKWENAFVSVYSKNNPNLLF
SMCGFEVRILPRQRMEEVVSNDEGVWDLVDERTKQRTAKAYLKVSEEEIK
KFDSRIRGILMASGSTTFTKVAAKWNTSLISLFTYFREAIVATEPLLDIL
VKGETRIQNRVKLGLNSKMPTRFPPAVFYTPKELGGLGMISASHILIPAS
DLSWSKQTDTGITHFRAGMTHEDEKLIPTIFRYITTWENEFLDSQRVWAE
YATKRQEAIQQNRRLAFEELEGSWDRGIPRISTLFQRDRHTLAYDRGHRI
RREFKQYSLERNSPFWWTNSHHDGKLWNLNAYRTDVIQALGGIETILEHT
LFKGTGFNSWEGLFWEKASGFEDSMQFKKLTHAQRTGLSQIPNRRFTLWW
SPTINRANVYVGFLVQLDLTGIFLHGKIPTLKISLIQIFRAHLWQKIHES
IVFDICQILDGELDVLQIESVTKETVHPRKSYKMNSSAADITMESVHEWE
VSKPSLLHETNDSFKGLITNKMWFDVQLRYGDYDSHDISRYVRAKFLDYT
TDNVSMYPSPTGVMIGIDLAYNMYDAYGNWFNGLKPLIQNSMRTIMKANP
ALYVLRERIRKGLQIYQSSVQEPFLNSSNYAELFNNDIKLFVDDTNVYRV
TVHKTFEGNVATKAINGCIFTLNPKTGHLFLKIIHTSVWAGQKRLSQLAK
WKTAEEVSALVRSLPKEEQPKQIIVTRKAMLDPLEVHMLDFPNIAIRPTE
LRLPFSAAMSIDKLSDVVMKATEPQMVLFNIYDDWLDRISSYTAFSRLTL
LLRALKTNEESAKMILLSDPTITIKSYHLWPSFTDEQWITIESQMRDLIL
TEYGRKYNVNISALTQTEIKDIILGNEWRKSAIANTLLYLRLKNIYVSAD
DFVEEQNVYVLPKNLLKKFIEISDVKIQVAAFIYGMSAKDHPKVKEIKTV
VLVPQLGHVGSVQISNIPDIGDLPDTEGLELLGWIHTQTEELKFMAASEV
ATHSKLFADKKRDCIDISIFSTPGSVSLSAYNLTDEGYQWGEENKDIMNV
LSEGFEPTFSTHAQLLLSDRITGNFIIPSGNVWNYTFMGTAFNQEGDYNF
KYGIPLEFYNEMHRPVHFLQFSELAGD
Ligand information
>5zwm Chain F (length=99) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
guucgcgaaauuuuacuucguggacauuuggucaauuugaaacaauacag
aaucagcaguuccccugcauaaggaugaaccgucaaagagauuuguuuu
<<<<<<<<<.......>>>>>>>>>.........<<<......>>>....
.................................................
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB5zwm Structures of the fully assembledSaccharomyces cerevisiaespliceosome before activation
Resolution3.4 Å
Binding residue
(original residue number in PDB)		R236 P515 V516 T607 K608 E609 R614 Y655 H659 Q662 Y667 R668 Y671 K681 K684 S1377 K1378 F1623 V1625 H1635 K1637 I1646 R1650 A1651 R1689 R1818
Binding residue
(residue number reindexed from 1)		R106 P367 V368 T459 K460 E461 R466 Y507 H511 Q514 Y519 R520 Y523 K533 K536 S1217 K1218 F1463 V1465 H1475 K1477 I1486 R1490 A1491 R1529 R1658
Enzymatic activity
Enzyme Commision number ?
Gene Ontology
Molecular Function
GO:0000386 second spliceosomal transesterification activity
GO:0003723 RNA binding
GO:0003729 mRNA binding
GO:0005515 protein binding
GO:0008233 peptidase activity
GO:0008237 metallopeptidase activity
GO:0017070 U6 snRNA binding
GO:0030619 U1 snRNA binding
GO:0030620 U2 snRNA binding
GO:0030623 U5 snRNA binding
GO:0097157 pre-mRNA intronic binding
Biological Process
GO:0000244 spliceosomal tri-snRNP complex assembly
GO:0000350 generation of catalytic spliceosome for second transesterification step
GO:0000387 spliceosomal snRNP assembly
GO:0000389 mRNA 3'-splice site recognition
GO:0000395 mRNA 5'-splice site recognition
GO:0000398 mRNA splicing, via spliceosome
GO:0006397 mRNA processing
GO:0008380 RNA splicing
Cellular Component
GO:0005634 nucleus
GO:0005681 spliceosomal complex
GO:0005682 U5 snRNP
GO:0005737 cytoplasm
GO:0046540 U4/U6 x U5 tri-snRNP complex
GO:0071013 catalytic step 2 spliceosome

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:5zwm, PDBe:5zwm, PDBj:5zwm
PDBsum5zwm
PubMed29794219
UniProtP33334|PRP8_YEAST Pre-mRNA-splicing factor 8 (Gene Name=PRP8)

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