Structure of PDB 5z6w Chain A Binding Site BS01

Receptor Information

>5z6w Chain A (length=546) Species: 208964 (Pseudomonas aeruginosa PAO1)

PALPEPFYYLHNFRAVLAWIGERYADLLDDQERAFIAAFAELPEASQALL
VRMVMRKGTLFREGKLAYAEIGDTRAAVQPLLALGWVDAQPTLELAQLFG
LLKKDELSQLFRDHLGRANLRKDALLERLQPLFPEARRLAEWQADFAEPV
YELRCMALCDRLRLMYFGNLWQDWSEFVLADLGIYRYESVEFSADSRGFR
LRADVDAYLHLFDCRQRFDLGEPLEELLAGLPGEPYANPWLEGRRVKLLF
QFAQHCEKQRDFDLAQRLYRQSSHPGARLRAIRSLERGERFAEAHALARE
ASCAPESDAERQGLARLLPRLQGKLGLPRQARAAAPEIDRLDLCLAFPSE
PCSVEWAVREHLEEPGCAVHYVENGLINSLFGLLCWEAIFAAIPGAFFHP
FHSAPADLHSADFRQRRAALFEACLGRLEDGSYRDAIRCRYRDKFGLQSP
FVYWELLGEELLEQALDCLPAAHLRAWFERLLEDIPGNRAGLPDLIQFWP
AQRRYRMVEVKGPGDRLQDNQLRWLQFCREREMPVAVCYVRWHVDD

Ligand information

>5z6w Chain H (length=10)

gttgggattg

Receptor-Ligand Complex Structure

Structure summary

• PDB: 5z6w Structural mechanism of DNA interstrand cross-link unhooking by the bacterial FAN1 nuclease.
• Resolution: 3.2 Å
• Binding residue (original residue number in PDB): A15 Y21 R65 R69 K70 Y81 L192
• Binding residue (residue number reindexed from 1): A2 Y8 R52 R56 K57 Y68 L179

Enzymatic activity

• Enzyme Commision number: 3.1.4.1: phosphodiesterase I.

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003676 nucleic acid binding
• GO:0004518 nuclease activity
• GO:0004519 endonuclease activity
• GO:0004527 exonuclease activity
• GO:0004528 phosphodiesterase I activity
• GO:0008409 5'-3' exonuclease activity
• GO:0016788 hydrolase activity, acting on ester bonds
• GO:0017108 5'-flap endonuclease activity
• GO:0030145 manganese ion binding
• GO:0046872 metal ion binding
• GO:0070336 flap-structured DNA binding

Biological Process

• GO:0006281 DNA repair
• GO:0036297 interstrand cross-link repair

External links

• PDB: RCSB:5z6w, PDBe:5z6w, PDBj:5z6w
• PDBsum: 5z6w
• PubMed: 29514982
• UniProt: Q9I2N0|FAN1_PSEAE Fanconi-associated nuclease 1 homolog (Gene Name=fan1)