Structure of PDB 5y36 Chain A Binding Site BS01

Receptor Information
>5y36 Chain A (length=1368) Species: 301447 (Streptococcus pyogenes serotype M1) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGA
LLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHR
LEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKAD
LRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENP
INASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTP
NFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAI
LLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEI
FFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY
YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDK
NLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVD
LLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKI
IKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQ
LKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDD
SLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKV
MGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHP
VENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDD
SIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNL
TKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLI
REVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKK
YPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEI
TLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEV
QTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVE
KGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPK
YSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPE
DNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDK
PIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQ
SITGLYETRIDLSQLGGD
Ligand information
>5y36 Chain B (length=99) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
gguaccgcuccagucguucaugguuuuagagcuagaaauagcaaguuaaa
auaaggcuaguccguuaucaacuugaaaaaguggcaccgagucggugcu
......................<<<<<<..<<<<....>>>>....>>>>
>>..<<.....>>.......<<<<....>>>>.<<<<<<...>>>>>>.
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB5y36 Structural insights into DNA cleavage activation of CRISPR-Cas9 system
Resolution5.2 Å
Binding residue
(original residue number in PDB)		S29 K30 R40 K44 K45 R63 R69 R70 Y72 R74 R75 K76 R78 Y81 F105 R115 V126 Y128 P133 T134 I135 L156 K163 F164 R165 G166 H167 Y325 K401 R403 T404 G408 R447 I448 Y450 A456 R457 N459 T472 I473 T474 N692 Q695 G729 K735 K772 K848 K1085 P1090 Q1091 V1100 T1102 G1103 G1104 F1105 E1108 K1130 Y1131 F1134 M1169 R1171 K1211 R1279 Y1356
Binding residue
(residue number reindexed from 1)		S29 K30 R40 K44 K45 R63 R69 R70 Y72 R74 R75 K76 R78 Y81 F105 R115 V126 Y128 P133 T134 I135 L156 K163 F164 R165 G166 H167 Y325 K401 R403 T404 G408 R447 I448 Y450 A456 R457 N459 T472 I473 T474 N692 Q695 G729 K735 K772 K848 K1085 P1090 Q1091 V1100 T1102 G1103 G1104 F1105 E1108 K1130 Y1131 F1134 M1169 R1171 K1211 R1279 Y1356
Enzymatic activity
Enzyme Commision number 3.1.-.-
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003723 RNA binding
GO:0004519 endonuclease activity
GO:0004520 DNA endonuclease activity
GO:0004527 exonuclease activity
GO:0008408 3'-5' exonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0043571 maintenance of CRISPR repeat elements
GO:0051607 defense response to virus

View graph for
Molecular Function
View graph for
Biological Process
External links
PDB RCSB:5y36, PDBe:5y36, PDBj:5y36
PDBsum5y36
PubMed29123204
UniProtQ99ZW2|CAS9_STRP1 CRISPR-associated endonuclease Cas9/Csn1 (Gene Name=cas9)

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