Structure of PDB 5vzl Chain A Binding Site BS01

Receptor Information
>5vzl Chain A (length=1364) Species: 160490 (Streptococcus pyogenes M1 GAS) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
KKYSIGLDIGTNSVGWAVITDDYKVPSKKFKVLGNTDRHSIKKNLIGALL
FDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLR
LIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPIN
ASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNF
KSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILL
SDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFF
DQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQ
RTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYV
GPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNL
PNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLL
FKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIK
DKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLK
RRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSL
TFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMG
RHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVE
NTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSI
DNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTK
AERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIRE
VKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYP
KLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITL
ANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQT
GGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKG
KSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYS
LFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDN
EQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPI
REQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSI
TGLYETRIDLSQLG
Ligand information
>5vzl Chain B (length=116) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
ggcgcauaaagaugagacgcguuuuagagcuaugcuguuuugaaaaaaac
agcauagcaaguuaaaauaaggcuaguccguuaucaacuugaaaaagugg
caccgagucggugcuu
....................<<<<<<..<<<<<<<<<<<.......>>.>
>>>>>>>>....>>>>>>..<<.....>>.......<<<<....>>>>.<
<<<<<...>>>>>>..
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB5vzl Disabling Cas9 by an anti-CRISPR DNA mimic.
Resolution3.9 Å
Binding residue
(original residue number in PDB)		K30 K33 L35 R40 I43 K44 N46 G56 E57 T58 A59 T62 K65 R66 R69 R70 R71 Y72 R74 R75 N77 Y81 L101 S104 F105 R115 H116 I122 H129 H160 F164 R165 G166 Y325 H328 H329 K336 R340 K348 F351 F352 Y359 K401 Q402 R403 T404 F405 N407 G408 F446 I448 Y450 L455 R457 N459 T472 I473 P475 M495 N497 R661 G729 K735 L738 A764 T893 R919 T924 K968 R976 M1089 P1090 V1100 T1102 G1103 G1104 F1105 I1110 K1113 R1122 K1123 K1124 M1169 E1225 L1226 A1227 Q1272 H1349 Q1350 S1351 Y1356 T1358
Binding residue
(residue number reindexed from 1)		K28 K31 L33 R38 I41 K42 N44 G54 E55 T56 A57 T60 K63 R64 R67 R68 R69 Y70 R72 R73 N75 Y79 L99 S102 F103 R113 H114 I120 H127 H158 F162 R163 G164 Y323 H326 H327 K334 R338 K346 F349 F350 Y357 K399 Q400 R401 T402 F403 N405 G406 F444 I446 Y448 L453 R455 N457 T470 I471 P473 M493 N495 R659 G727 K733 L736 A762 T891 R917 T922 K966 R974 M1087 P1088 V1098 T1100 G1101 G1102 F1103 I1108 K1111 R1120 K1121 K1122 M1167 E1223 L1224 A1225 Q1270 H1347 Q1348 S1349 Y1354 T1356
Enzymatic activity
Enzyme Commision number 3.1.-.-
Gene Ontology
Molecular Function
GO:0003676 nucleic acid binding
GO:0003677 DNA binding
GO:0003723 RNA binding
GO:0004519 endonuclease activity
GO:0004520 DNA endonuclease activity
GO:0004527 exonuclease activity
GO:0008408 3'-5' exonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0043571 maintenance of CRISPR repeat elements
GO:0051607 defense response to virus

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Molecular Function
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Biological Process
External links
PDB RCSB:5vzl, PDBe:5vzl, PDBj:5vzl
PDBsum5vzl
PubMed28706995
UniProtQ99ZW2|CAS9_STRP1 CRISPR-associated endonuclease Cas9/Csn1 (Gene Name=cas9)

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