Structure of PDB 5oxj Chain A Binding Site BS01

Receptor Information

>5oxj Chain A (length=532) Species: 271 (Thermus aquaticus)

ALEEAPWPPPEGAFVGFVLSRKEPMWADLLALAAARGGRVHRAPEPYKAL
RDLKEARGLLAKDLSVLALREGLGLPPGDDPMLLAYLLDPSNTTPEGVAR
RYGGEWTEEAGERAALSERLFANLWGRLEGEERLLWLYREVERPLSAVLA
HMEATGVRLDVAYLRALSLEVAEEIARLEAEVFRLAGHPFNLNSRDQLER
VLFDELGLPAIGKTEKTGKRSTSAAVLEALREAHPIVEKILQYRELTKLK
STYIDPLPDLIHPRTGRLHTRFNQTATATGRLSSSDPNLQNIPVRTPLGQ
RIRRAFIAEEGWLLVALDYSQIELRVLAHLSGDENLIRVFQEGRDIHTET
ASWMDPLMRRAAKTINFGVLYGMSAHRLSQELAIPYEEAQAFIERYFQSF
PKVRAWIEKTLEEGRRRGYVETLFGRRRYVPDLEARVKSVREAAERKAFN
MPVQGTAADLMKLAMVKLFPRLEEMGARMLLQVHDELVLEAPKERAEAVA
RLAKEVMEGVYPLAVPLEVEVGIGEDWLSAKE

Ligand information

>5oxj Chain B (length=12)

gaccacggcgcg

Receptor-Ligand Complex Structure

Structure summary

• PDB: 5oxj Structural basis for the selective incorporation of an artificial nucleotide opposite a DNA adduct by a DNA polymerase.
• Resolution: 2.0 Å
• Binding residue (original residue number in PDB): R487 T506 K508 T509 S513 T514 S515 A516 R536 K540 Y545 R573 N583 I584 P585 V586 R587 V783 H784
• Binding residue (residue number reindexed from 1): R195 T214 K216 T217 S221 T222 S223 A224 R244 K248 Y253 R281 N291 I292 P293 V294 R295 V483 H484

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Gene Ontology

Molecular Function

• GO:0001882 nucleoside binding
• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity

Biological Process

• GO:0006260 DNA replication
• GO:0006261 DNA-templated DNA replication
• GO:0006281 DNA repair

External links

• PDB: RCSB:5oxj, PDBe:5oxj, PDBj:5oxj
• PDBsum: 5oxj
• PubMed: 29136072
• UniProt: P19821|DPO1_THEAQ DNA polymerase I, thermostable (Gene Name=polA)