Structure of PDB 5on2 Chain A Binding Site BS01

Receptor Information
>5on2 Chain A (length=862) Species: 83333 (Escherichia coli K-12) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
SHMQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYPSGRL
HMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPA
PWTYDNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGL
VYKKTSAVNWCPNDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAY
ADELLNDLDKLDHWPDTVKTMQRNWIGRSEGVEITFNVNDYDNTLTVYTT
RPDAFMGCTYLAVAAGHPLAQKAAENNPELAAFIDECRNTKVAEAEMATM
EKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGAVMAVPGHDQRDYEF
ASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDHEAAFNA
IADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDD
QLPVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSW
YYARYTCPQYKEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLM
RDAGMVNSDEPAKQLLCQGMVLADAFYYVGENGERNWVSPVDAIVERDEK
GRIVKAKDAAGHELVYTGMSKMSKSKNNGIDPQVMVERYGADTVRLFMMF
ASPADMTLEWQESGVEGANRFLKRVWKLVYEHTAKGDVAALNVDALTENQ
KALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAPTDGEQDRA
LMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVI
YVPGKLLNLVVG
Ligand information
>5on2 Chain B (length=85) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
gcccggaugguggaaucgguagacacaagggauuuaaaaucccucggcgc
gcgcugugcggguucaagucccgcuccggguacca
<<<<<<<..<<<...........>>>.<<<<<<<...>>>>>>>.<<<<.
.>>>>..<<<<<.......>>>>>>>>>>>>....
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB5on2 Kinetic Origin of Substrate Specificity in Post-Transfer Editing by Leucyl-tRNA Synthetase.
Resolution3.1 Å
Binding residue
(original residue number in PDB)		Y43 D80 G83 L84 A167 N168 E169 T215 M219 N222 W223 E292 A296 T297 R416 R418 R424 F493 E532 H533 M617 S618 K619 F648 A649 S650 Q659 S661 G665 R668 K711 R719 N723 T724 M730 E731 N734 R811 K813 Y838 V850 L854 N856
Binding residue
(residue number reindexed from 1)		Y45 D82 G85 L86 A169 N170 E171 T217 M221 N224 W225 E294 A298 T299 R418 R420 R426 F495 E534 H535 M619 S620 K621 F650 A651 S652 Q661 S663 G667 R670 K713 R721 N725 T726 M732 E733 N736 R813 K815 Y840 V852 L856 N858
Enzymatic activity
Catalytic site (original residue number in PDB) L41 H49 H52 D80 C159 D162 C176 C179 F493 H533 F543 K619 K622
Catalytic site (residue number reindexed from 1) L43 H51 H54 D82 C161 D164 C178 C181 F495 H535 F545 K621 K624
Enzyme Commision number 6.1.1.4: leucine--tRNA ligase.
Gene Ontology
Molecular Function
GO:0000166 nucleotide binding
GO:0002161 aminoacyl-tRNA editing activity
GO:0004812 aminoacyl-tRNA ligase activity
GO:0004823 leucine-tRNA ligase activity
GO:0005515 protein binding
GO:0005524 ATP binding
Biological Process
GO:0006412 translation
GO:0006418 tRNA aminoacylation for protein translation
GO:0006429 leucyl-tRNA aminoacylation
GO:0106074 aminoacyl-tRNA metabolism involved in translational fidelity
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:5on2, PDBe:5on2, PDBj:5on2
PDBsum5on2
PubMed29111343
UniProtP07813|SYL_ECOLI Leucine--tRNA ligase (Gene Name=leuS)

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