Structure of PDB 5o9z Chain A Binding Site BS01

Receptor Information
>5o9z Chain A (length=2190) Species: 9606 (Homo sapiens) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
PEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQ
IRDVPVLYHITGAISFVNEIPWVIEPVYISQWGSMWIMMRREKRDRRHFK
RMRFPPFDDEEPPLDYADNILDVEPLEAIQLELDPEEDAPVLDWFYDHQP
LRDSRKYVNGSTYQRWQFTLPMMSTLYRLANQLLTDLVDDNYFYLFDLKA
FFTSKALNMAIPGGPKFEPLVRDINLQDEDWNEFNDINKIIIRQPIRTEY
KIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPISHR
HSVKSQEPLPDDDEEFELPEFVEPFLKDTPLYTDNTANGIALLWAPRPFN
LRSGRTRRALDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHR
PPKAQKKRYLFRSFKATKFFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKN
LNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREVLRLTKLVVDSHV
QYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMRQIRMCKDLKHLI
YYRFNTGPVGKGPGCGFWAAGWRVWLFFMRGITPLLERWLGNLLARQFEV
ESHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCWKANIPWK
VPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLG
RLTRLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPF
PPLSYKHDTKLLILALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEA
LSRIKRHLLTQRAFKEVGIEFMDLYSHLVPVYDVEPLEKITDAYLDQYLW
YEADKRRLFPPWIKPADTEPPPLLVYKWCQGINNLQDVWETSEGECNVML
ESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNS
YGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDI
ATEAAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEHPDPNNENIVG
YNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSV
YSKDNPNLLFNMCGFECRILPKCRTSYEEFTHKDGVWNLQNEVTKERTAQ
CFLRVDDESMQRFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREA
VVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPKELGGLGM
LSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWES
EFIDSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDR
HTLAYDKGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQA
LGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKWKKLTNAQRSGLN
QIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIF
RAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSYKMNSSCA
DILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIE
RYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQ
QAMAKIMKANPALYVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQII
WFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVW
AGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHLL
DFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLYDDWLKTI
SSYTAFSRLILILRALHVNNDRAKVILKPDKTTITEPHHIWPTLTDEEWI
KVEVQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISKTEWRVRAIS
AANLHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICISDLRAQIAGYLY
GVSPPDNPQVKEIRCIVMVPQWGTHQTVHLPGQLPQHEYLKEMEPLGWIH
TQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTPGSCTLTAYK
LTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSW
NYNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFAL
Ligand information
>5o9z Chain Y (length=46) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
ccuccgaacgguaagagccuagcauguugaugucauacuuauccug
..............................................
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB5o9z Cryo-EM Structure of a Pre-catalytic Human Spliceosome Primed for Activation.
Resolution4.5 Å
Binding residue
(original residue number in PDB)		Y592 M599 G1550 Q1552 K1565
Binding residue
(residue number reindexed from 1)		Y530 M537 G1473 Q1475 K1488
Enzymatic activity
Enzyme Commision number ?
Gene Ontology
Molecular Function
GO:0003723 RNA binding
GO:0005515 protein binding
GO:0008233 peptidase activity
GO:0008237 metallopeptidase activity
GO:0017070 U6 snRNA binding
GO:0030619 U1 snRNA binding
GO:0030620 U2 snRNA binding
GO:0030623 U5 snRNA binding
GO:0070530 K63-linked polyubiquitin modification-dependent protein binding
GO:0097157 pre-mRNA intronic binding
GO:0140492 metal-dependent deubiquitinase activity
Biological Process
GO:0000244 spliceosomal tri-snRNP complex assembly
GO:0000375 RNA splicing, via transesterification reactions
GO:0000398 mRNA splicing, via spliceosome
GO:0006397 mRNA processing
GO:0008380 RNA splicing
GO:0071222 cellular response to lipopolysaccharide
GO:0071356 cellular response to tumor necrosis factor
Cellular Component
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005681 spliceosomal complex
GO:0005682 U5 snRNP
GO:0016020 membrane
GO:0016607 nuclear speck
GO:0030532 small nuclear ribonucleoprotein complex
GO:0046540 U4/U6 x U5 tri-snRNP complex
GO:0071005 U2-type precatalytic spliceosome
GO:0071006 U2-type catalytic step 1 spliceosome
GO:0071007 U2-type catalytic step 2 spliceosome
GO:0071013 catalytic step 2 spliceosome

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:5o9z, PDBe:5o9z, PDBj:5o9z
PDBsum5o9z
PubMed28781166
UniProtQ6P2Q9|PRP8_HUMAN Pre-mRNA-processing-splicing factor 8 (Gene Name=PRPF8)

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