Structure of PDB 5n8o Chain A Binding Site BS01

Receptor Information
>5n8o Chain A (length=1432) Species: 562 (Escherichia coli) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGKGAEQLGLQGSVDKD
VFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDK
RLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSR
DQEPQLHTHAVVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLY
REKLKEQVEALGYETEVVGKHGMWEMPGVPVEAFSVDPEIRMAEWMQTLK
ETGFDIRAYRDAADQRTEIRTQAPDVQQAVTQAIAGLSERKVQFTYTDVL
ARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDEL
SVRALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQG
GAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQR
TGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRNVRYARLAGDFAAS
VKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVTMTALSPVWLD
SRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGET
QVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
SEDAMTVVVPGRAEPASLPVSDSPFTALKLENGWVETPGHSVSDSATVFA
SVTQMAMNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIK
ARAGETLLETAISLQKAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEA
KSFAAEGTGFTELGGEINAQIKRGDLLYVDVAKGYGTGLLVSRASYEAEK
SILRHILEGKEAVTPLMERVPGELMETLTSGQRAATRMILETSDRFTVVQ
GYAGVGKTTQFRAVMSAVNMLPASERPRVVGLGPTHRAVGEMRSAGVDAQ
TLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTEMARAYALIAAGG
GRAVASGDTDQLQAIAPGQSFRLQQTRSAADVVIMKEIVRQTPELREAVY
SLINRDVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQ
QKAMLKGEAFPDIPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLN
SMIHDAREKAGELGKEQVMVPVLNTANIRDGELRRLSTWEKNPDALALVD
NVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVTLYTPDKIRVGT
GDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAE
QHIDLAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQ
HVQVYTDNRQGWTDAINNAVQKGTAHDVLEPK
Ligand information
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB5n8o Cryo-EM Structure of a Relaxase Reveals the Molecular Basis of DNA Unwinding during Bacterial Conjugation
Resolution3.9 Å
Binding residue
(original residue number in PDB)		K179 Y190 I194 H221 Q333 D469 R470 T543 W638 T721 G722 K723 M795 S832 F833 P1025 T1026 H1027 A1044 S1045 H1048 A1105 I1106 L1233 R1352 H1403 R1423 K1424 M1426
Binding residue
(residue number reindexed from 1)		K179 Y190 I194 H221 Q293 D429 R430 T503 W598 T681 G682 K683 M755 S791 F792 P984 T985 H986 A1003 S1004 H1007 A1064 I1065 L1192 R1311 H1362 R1382 K1383 M1385
Enzymatic activity
Enzyme Commision number 3.6.4.12: DNA helicase.
5.6.2.1: DNA topoisomerase.
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003678 DNA helicase activity
GO:0003917 DNA topoisomerase type I (single strand cut, ATP-independent) activity
GO:0004386 helicase activity
GO:0005524 ATP binding
GO:0016787 hydrolase activity
GO:0016818 hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
GO:0016853 isomerase activity
GO:0016887 ATP hydrolysis activity
GO:0046872 metal ion binding
Biological Process
GO:0032508 DNA duplex unwinding
Cellular Component
GO:0005737 cytoplasm

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:5n8o, PDBe:5n8o, PDBj:5n8o
PDBsum5n8o
PubMed28457609
UniProtP14565|TRAI1_ECOLI Multifunctional conjugation protein TraI (Gene Name=traI)

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