Structure of PDB 5i8q Chain A Binding Site BS01

Receptor Information
>5i8q Chain A (length=752) Species: 559292 (Saccharomyces cerevisiae S288C) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
KRRFSSEHPDPVETSIPEQAAEIAEELSKQHPLPSEEPLVHHDAGEFKGL
QRHHTSAEEAQKLEDGKINPFTGREFTPKYVDILKIRRELPVHAQRDEFL
KLYQNNQIMVFVGETGSGKTTQIPQFVLFDEMPHLENTQVACTQPRRVAA
MSVAQRVAEEMDVKLGEEVGYSIRFENKTSNKTILKYMTDGMLLREAMED
HDLSRYSCIILDEAHERTLATDILMGLLKQVVKRRPDLKIIIMSATLDAE
KFQRYFNDAPLLAVPGRTYPVELYYTPEFQRDYLDSAIRTVLQIHATEEA
GDILLFLTGEDEIEDAVRKISLEGDQLVREEGCGPLSVYPLYGSLPPHQQ
QRIFEPAPESHNGRPGRKVVISTNIAETSLTIDGIVYVVDPGFSKQKVYN
PRIRVESLLVSPISKASAQQRAGRAGRTRPGKCFRLYTEEAFQKELIEQS
YPEILRSNLSSTVLELKKLGIDDLVHFDFMDPPAPETMMRALEELNYLAC
LDDEGNLTPLGRLASQFPLDPMLAVMLIGSFEFQCSQEILTIVAMLSVPN
VFIRPTKDKKRADDAKNIFAHPDGDHITLLNVYHAFKSDEAYEYGIHKWC
RDHYLNYRSLSAADNIRSQLERLMNRYNLELNTTDYESPKYFDNIRKALA
SGFFMQVAKKRSGAKGYITVKDNQDVLIHPSTVLGHDAEWVIYNEFVLTS
KNYIRTVTSVRPEWLIEIAPAYYDLSNFQKGDVKLSLERIKEKVDRLNEL
KQ
Ligand information
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB5i8q Structure of the DEAH/RHA ATPase Prp43p bound to RNA implicates a pair of hairpins and motif Va in translocation along RNA.
Resolution4.2 Å
Binding residue
(original residue number in PDB)		R150 R177 T192 G194 M195 R198 E199 Y345 G346 N377 K398 L411 P552 Q622 R625 R629 T702
Binding residue
(residue number reindexed from 1)		R147 R174 T189 G191 M192 R195 E196 Y342 G343 N374 K395 L408 P549 Q619 R622 R626 T699
Enzymatic activity
Enzyme Commision number 3.6.4.13: RNA helicase.
Gene Ontology
Molecular Function
GO:0003676 nucleic acid binding
GO:0003723 RNA binding
GO:0003724 RNA helicase activity
GO:0003729 mRNA binding
GO:0004386 helicase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0008186 ATP-dependent activity, acting on RNA
GO:0016787 hydrolase activity
GO:0016887 ATP hydrolysis activity
Biological Process
GO:0000390 spliceosomal complex disassembly
GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
GO:0000466 maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
GO:0006364 rRNA processing
GO:0006396 RNA processing
GO:0006397 mRNA processing
GO:0008380 RNA splicing
GO:0022613 ribonucleoprotein complex biogenesis
GO:0030490 maturation of SSU-rRNA
GO:0042273 ribosomal large subunit biogenesis
Cellular Component
GO:0005634 nucleus
GO:0005681 spliceosomal complex
GO:0005730 nucleolus
GO:0005739 mitochondrion
GO:0030686 90S preribosome
GO:0032040 small-subunit processome
GO:0071014 post-mRNA release spliceosomal complex
GO:1990904 ribonucleoprotein complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:5i8q, PDBe:5i8q, PDBj:5i8q
PDBsum5i8q
PubMed28416566
UniProtP53131|PRP43_YEAST Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP43 (Gene Name=PRP43)

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