Structure of PDB 5ekg Chain A Binding Site BS01

Receptor Information

>5ekg Chain A (length=425) Species: 10090 (Mus musculus)

GTVNWSVEDIVKGINSNNLESQLQATQAARKLLSREKQPPIDNIIRAGLI
PKFVSFLGKTDCSPIQFESAWALTNIASGTSEQTKAVVDGGAIPAFISLL
ASPHAHISEQAVWALGNIAGDGSAFRDLVIKHGAIDPLLALLAVPDLSTL
ACGYLRNLTWTLSNLCRNKNPAPPLDAVEQILPTLVRLLHHNDPEVLADS
CWAISYLTDGPNERIEMVVKKGVVPQLVKLLGATELPIVTPALRAIGNIV
TGTDEQTQKVIDAGALAVFPSLLTNPKTNIQKEATWTMSNITAGRQDQIQ
QVVNHGLVPFLVGVLSKADFKTQKEAAWAITNYTSGGTVEQIVYLVHCGI
IEPLMNLLSAKDTKIIQVILDAISNIFQAAEKLGETEKLSIMIEECGGLD
KIEALQRHENESVYKASLNLIEKYF

Ligand information

>5ekg Chain B (length=5) Species: 32630 (synthetic construct)

RKRKT

Receptor-Ligand Complex Structure

Structure summary

• PDB: 5ekg Structural and Calorimetric Studies Demonstrate that Xeroderma Pigmentosum Type G (XPG) Can Be Imported to the Nucleus by a Classical Nuclear Import Pathway via a Monopartite NLS Sequence.
• Resolution: 2.8 Å
• Binding residue (original residue number in PDB): D280 G281 N283 V321 T322 W357 N361 A364 E396 W399 S406
• Binding residue (residue number reindexed from 1): D209 G210 N212 V250 T251 W286 N290 A293 E325 W328 S335

Enzymatic activity

• Catalytic site (original residue number in PDB): T151 A163 N361 R366 Q367
• Catalytic site (residue number reindexed from 1): T80 A92 N290 R295 Q296

Gene Ontology

Molecular Function

• GO:0061608 nuclear import signal receptor activity

Biological Process

• GO:0006606 protein import into nucleus

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:5ekg, PDBe:5ekg, PDBj:5ekg
• PDBsum: 5ekg
• PubMed: 26812207
• UniProt: P52293|IMA1_MOUSE Importin subunit alpha-1 (Gene Name=Kpna2)