Structure of PDB 5aa1 Chain A Binding Site BS01

Receptor Information

>5aa1 Chain A (length=418) Species: 1402513 (Pseudomonas aeruginosa BWHPSA013)

EGVLRVITRNSPATYFQDRNGETGFEYELAKRFAERLGVELKIETADNLD
DLYAQLSREGGPALAAAGLTPGREDDASVRYSHTYLDVTPQIIYRNGQQR
PTRPEDLVGKRIMVLKGSSHAEQLAELKKQYPELKYEESDAVEVVDLLRM
VDVGDIDLTLVDSNELAMNQVYFPNVRVAFDFGEARGLAWALPGGDDDSL
MNEVNAFLDQAKKEGLLQRLKDRYYGHVDVLGYVGAYTFTQHLQQRLPRY
ESHFKQSGKQKDTDWRLLAAIGYQESLWQPGATSKTGVRGLMMLTNRTAQ
AMGVSNRLDPKQSIQGGSKYFVQIRSELPESIKEPDRSWFALAAYNIGGA
HLEDARKMAEKEGLNPNKWLDVKKMLPRLAQKQWYAKTRYGYARGGETVH
FVQNVRRYYDILTWVTQP

Ligand information

>5aa1 Chain E (length=6) Species: 287 (Pseudomonas aeruginosa)

AAEKAA

Receptor-Ligand Complex Structure

Structure summary

• PDB: 5aa1 Activation by Allostery in Cell-Wall Remodeling by a Modular Membrane-Bound Lytic Transglycosylase from Pseudomonas aeruginosa.
• Resolution: 2.89 Å
• Binding residue (original residue number in PDB): R50 A54 F66 E67 Y94 G109 L110 T111 V155 L156 G158 S159 S160 H161 E163 V185 L201 V202 D203 N205 E206 L229
• Binding residue (residue number reindexed from 1): R9 A13 F25 E26 Y53 G68 L69 T70 V114 L115 G117 S118 S119 H120 E122 V144 L160 V161 D162 N164 E165 L188

Enzymatic activity

• Enzyme Commision number: 4.2.2.n1: peptidoglycan lytic exotransglycosylase.

Gene Ontology

Molecular Function

• GO:0008933 lytic transglycosylase activity
• GO:0016837 carbon-oxygen lyase activity, acting on polysaccharides

Biological Process

• GO:0000270 peptidoglycan metabolic process

Cellular Component

• GO:0016020 membrane

External links

• PDB: RCSB:5aa1, PDBe:5aa1, PDBj:5aa1
• PDBsum: 5aa1
• PubMed: 27618662
• UniProt: Q9HXN1|MLTF_PSEAE Membrane-bound lytic murein transglycosylase F (Gene Name=mltF)