Structure of PDB 4x4s Chain A Binding Site BS01

Receptor Information
>4x4s Chain A (length=443) Species: 224325 (Archaeoglobus fulgidus DSM 4304) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
MKVEEILEKALELVIPDEEEVRKGREAEEELRRRLDELGVEYVFVGSYAR
NTWLKGSLEIDVFLLFPEEFSKEELRERGLEIGKAVLDSYEIRYAEHPYV
HGVVKGVEVDVVPCYKLKEPKNIKSAVDRTPFHHKWLEGRIKGKENEVRL
LKGFLKANGIYGAEYKVRGFSGYLCELLIVFYGSFLETVKNARRWTRRTV
IDVAKGEVRKGEEFFVVDPVDEKRNVAANLSLDNLARFVHLCREFMEAPS
LGFFKPKHPLEIEPERLRKIVEERGTAVFAVKFRKPDIVDDNLYPQLERA
SRKIFEFLERENFMPLRSAFKASEEFCYLLFECQIKEISRVFRRMGPQFE
DERNVKKFLSRNRAFRPFIENGRWWAFEMRKFTTPEEGVRSYASTHWHTL
GKNVGESIREYFEIISGEKLFKEPVTAELCEMMGVKDSNSSSV
Ligand information
>4x4s Chain B (length=35) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
ggccgcggcagguucgaguccugccgcgcgccacc
<<<<<<<<<<<<.......>>>>>>>>>.>>>...
Receptor-Ligand Complex Structure
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PDB4x4s On-enzyme refolding permits small RNA and tRNA surveillance by the CCA-adding enzyme.
Resolution3.25 Å
Binding residue
(original residue number in PDB)		A95 E96 H97 Y99 V112 V127 A163 Y165 R224 A228 N229 D291 N292 P295 Q296 R299 K303 N354 F358 R361 H396 H398 T399 K402
Binding residue
(residue number reindexed from 1)		A95 E96 H97 Y99 V112 V127 A163 Y165 R224 A228 N229 D291 N292 P295 Q296 R299 K303 N354 F358 R361 H396 H398 T399 K402
Enzymatic activity
Enzyme Commision number 2.7.7.72: CCA tRNA nucleotidyltransferase.
Gene Ontology
Molecular Function
GO:0000049 tRNA binding
GO:0000287 magnesium ion binding
GO:0003723 RNA binding
GO:0004810 CCA tRNA nucleotidyltransferase activity
GO:0005524 ATP binding
GO:0016779 nucleotidyltransferase activity
GO:0046872 metal ion binding
GO:0160016 CCACCA tRNA nucleotidyltransferase activity
Biological Process
GO:0001680 tRNA 3'-terminal CCA addition
GO:0008033 tRNA processing
GO:0031123 RNA 3'-end processing
GO:0042245 RNA repair
GO:0106354 tRNA surveillance

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Molecular Function
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Biological Process
External links
PDB RCSB:4x4s, PDBe:4x4s, PDBj:4x4s
PDBsum4x4s
PubMed25640237
UniProtO28126|CCA_ARCFU CCA-adding enzyme (Gene Name=cca)

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