Structure of PDB 4tvx Chain A Binding Site BS01

Receptor Information

>4tvx Chain A (length=192) Species: 83333 (Escherichia coli K-12)

MYLSKVIIARAWSRDLYQLHQGLWHLFPDFLFHVEKRNTPEGCHVLLQSA
QMPVSTAVATVIKTKQVEFQLQVGVPLYFRLRANPIKTILDNQKRLDSKG
NIKRCRVPLIKEAEQIAWLQRKLGNAARVEDVHPISERPQYFSGDGKSGK
IQTVCFEGVLTINDAPALIDLVQQGIGPAKSMGCGLLSLAPL

Ligand information

>4tvx Chain L (length=61)

auaaaccgacgguauuguucagauccuggcuugccaacaggaguuccccg
cgccagcgggg

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Receptor-Ligand Complex Structure

Structure summary

• PDB: 4tvx Structural biology. Crystal structure of the CRISPR RNA-guided surveillance complex from Escherichia coli.
• Resolution: 3.24 Å
• Binding residue (original residue number in PDB): Y17 N91 K94 T95 I96 D98 N99 Q100 R102 K110 R111 C112 R113 V114 P115 R128 K129 F149 S155 G156 K157 Q159 K187 S188
• Binding residue (residue number reindexed from 1): Y17 N84 K87 T88 I89 D91 N92 Q93 R95 K103 R104 C105 R106 V107 P108 R121 K122 F142 S148 G149 K150 Q152 K180 S181

Enzymatic activity

• Enzyme Commision number: 3.1.-.-

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0004519 endonuclease activity
• GO:0004521 RNA endonuclease activity
• GO:0005515 protein binding

Biological Process

• GO:0006396 RNA processing
• GO:0051607 defense response to virus
• GO:0099048 CRISPR-cas system

Cellular Component

• GO:0032991 protein-containing complex

External links

• PDB: RCSB:4tvx, PDBe:4tvx, PDBj:4tvx
• PDBsum: 4tvx
• PubMed: 25103409
• UniProt: Q46897|CAS6_ECOLI CRISPR system Cascade subunit CasE (Gene Name=casE)